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Literature summary for 4.2.2.3 extracted from

  • Preston, L.A.; Wong, T.Y.; Bender, C.L.; Schiller, N.L.
    Characterization of alginate lyase from Pseudomonas syringae pv. syringae (2000), J. Bacteriol., 182, 6268-6271.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.3

Activating Compound

Activating Compound Comment Organism Structure
NaCl 0.2 M Pseudomonas syringae

Cloned(Commentary)

Cloned (Comment) Organism
gene algL, DNA sequence determination and analysis, overexpression in Escherichia coli BL21(DE3), periplasmic enzyme location Pseudomonas syringae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, SDS-PAGE Pseudomonas syringae

Organism

Organism UniProt Comment Textmining
Pseudomonas syringae Q9L7P2 pv. syringae
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Pseudomonas syringae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity of different enzyme expressing plasmid constructs in Escherichia coli Pseudomonas syringae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deacetylated polymannuronic acid
-
 Pseudomonas syringae ?
-
 ?
additional information substrate specificity, residues His204 and Trp207 are critical for enzyme activity Pseudomonas syringae ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Pseudomonas syringae

Synonyms

Synonyms Comment Organism
alginate lyase
-
 Pseudomonas syringae
AlgL
-
 Pseudomonas syringae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
 Pseudomonas syringae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pseudomonas syringae