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Literature summary for 4.2.2.3 extracted from

  • Iwamoto, Y.; Araki, R.; Iriyama, K.; Oda, T.; Fukuda, H.; Hayashida, S.; Muramatsu, T.
    Purification and characterization of bifunctional alginate lyase from Alteromonas sp. strain no. 272 and its action on saturated oligomeric substrates (2001), Biosci. Biotechnol. Biochem., 65, 133-142.
    View publication on PubMed
Search for more literature for 4.2.2.3

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Alteromonas sp.
Cd2+
-
 Alteromonas sp.
Ni2+
-
 Alteromonas sp.
Zn2+
-
 Alteromonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 nona-alpha1,4-L-guluronate pH 8.5, 30°C Alteromonas sp.
0.028
-
 octa-alpha1,4-L-guluronate pH 8.5, 30°C Alteromonas sp.
0.031
-
 hepta-alpha1,4-L-guluronate pH 8.5, 30°C Alteromonas sp.
0.035
-
 hexa-alpha1,4-L-guluronate pH 8.5, 30°C Alteromonas sp.
0.039
-
 penta-alpha1,4-L-guluronate pH 8.5, 30°C Alteromonas sp.
0.172
-
 nona-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
0.186
-
 octa-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
0.192
-
 hepta-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
0.226
-
 hexa-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
0.232
-
 penta-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
3.86
-
 tetra-alpha1,4-L-guluronate pH 8.5, 30°C Alteromonas sp.
4.25
-
 tetra-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion to the culture medium Alteromonas sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Alteromonas sp.
K+ slightly activating Alteromonas sp.
Mg2+ slightly activating Alteromonas sp.
Mn2+ slightly activating Alteromonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 gel filtration Alteromonas sp.
33900
-
 1 * 33900, SDS-PAGE Alteromonas sp.

Organism

Organism UniProt Comment Textmining
Alteromonas sp.
-
 bifunctional enzyme, mannuronate- and guluronate-specific alginate lyase
-
Alteromonas sp. 272
-
 bifunctional enzyme, mannuronate- and guluronate-specific alginate lyase
-

Purification (Commentary)

Purification (Comment) Organism
from the culture medium, 135fold, to homogeneity Alteromonas sp.

Reaction

Reaction Comment Organism Reaction ID
R2-beta-D-mannuronic acid-R1 = R2-OH + 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-R1 enzyme seems to have 6 substrate binding sites, also performs reaction of EC 4.2.2.11 Alteromonas sp.
a

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Alteromonas sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hepta-alpha1,4-L-guluronate
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
hepta-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
hepta-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 272 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
hepta-beta1,4-D-mannuronic acid
-
 Alteromonas sp. D-mannuronic acid
-
 ?
hexa-alpha1,4-L-guluronate
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
hexa-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
hexa-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 272 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
hexa-beta1,4-D-mannuronic acid
-
 Alteromonas sp. D-mannuronic acid
-
 ?
additional information no activity with triguluronate and trimannuronate, but with tetramers or substrates with longer chain length Alteromonas sp. ?
-
 ?
additional information no activity with triguluronate and trimannuronate, but with tetramers or substrates with longer chain length Alteromonas sp. 272 ?
-
 ?
nona-alpha1,4-L-guluronate
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
nona-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
nona-beta1,4-D-mannuronic acid
-
 Alteromonas sp. D-mannuronic acid
-
 ?
octa-alpha1,4-L-guluronate
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
octa-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
octa-beta1,4-D-mannuronate
-
 Alteromonas sp. D-mannuronic acid
-
 ?
octa-beta1,4-D-mannuronic acid
-
 Alteromonas sp. D-mannuronic acid
-
 ?
octa-beta1,4-D-mannuronic acid
-
 Alteromonas sp. D-mannuronic acid + ?
-
 ?
penta-alpha1,4-L-guluronate
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
penta-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
penta-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 272 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
penta-beta1,4-D-mannuronic acid
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-beta-1,4-oligo-D-mannuronate + D-mannuronate
-
 ?
poly-alpha1,4-L-guluronic acid competitive to poly-beta1,4-D-mannuronic acid Alteromonas sp. ?
-
 ?
poly-beta1,4-D-mannuronic acid competitive to poly-alpha1,4L-guluronic acid Alteromonas sp. ?
-
 ?
sodium alginate endo-type manner Alteromonas sp. ?
-
 ?
tetra-alpha1,4-L-guluronate
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
tetra-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
tetra-alpha1,4-L-guluronic acid
-
 Alteromonas sp. 272 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate
-
 ?
tetra-beta1,4-D-mannuronic acid
-
 Alteromonas sp. 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-beta-1,4-D-mannuronoyl-beta-1,4-D-mannuranote + D-mannuronate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33900, SDS-PAGE Alteromonas sp.

Synonyms

Synonyms Comment Organism
alginate lyase
-
 Alteromonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Alteromonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
-
 Alteromonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5
-
 tetra-alpha1,4-L-guluronic acid pH 8.5, 30°C Alteromonas sp.
3.2
-
 tetra-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
6.2
-
 penta-alpha1,4-L-guluronic acid pH 8.5, 30°C Alteromonas sp.
7.3
-
 octa-alpha1,4-L-guluronic acid pH 8.5, 30°C Alteromonas sp.
7.4
-
 penta-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
7.6
-
 hepta-alpha1,4-L-guluronic acid pH 8.5, 30°C Alteromonas sp.
7.8
-
 nona-alpha1,4-L-guluronic acid pH 8.5, 30°C Alteromonas sp.
7.9
-
 hexa-alpha1,4-L-guluronic acid pH 8.5, 30°C Alteromonas sp.
10.8
-
 nona-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
11.1
-
 octa-beta1,4-D-mannuronic acid pH 8.5, 30°C Alteromonas sp.
11.9
-
 hexa-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.
13.2
-
 hepta-beta1,4-D-mannuronate pH 8.5, 30°C Alteromonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Alteromonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudoalteromonas elyakovii isoelectric focusing
-
 3.8