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Literature summary for 4.2.2.3 extracted from

  • Romeo, T.; Preston II, J.
    Purification and structural properties of an extracellular, (1-4)-beta-D-mannuronan-specific alginate lyase from a marine bacterium (1986), Biochemistry, 25, 8385-8391.
No PubMed abstract available
Search for more literature for 4.2.2.3

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 uncultured marine bacterium
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information requires significant levels of salt for maximal activity. Optimal activity at approximately 0.05 M with divalent cation and between 0.3 and 0.4 M with monovalent cation uncultured marine bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 gel filtration uncultured marine bacterium

Organism

Organism UniProt Comment Textmining
uncultured marine bacterium
-
 fermentative
-

Purification (Commentary)

Purification (Comment) Organism
-
 uncultured marine bacterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 uncultured marine bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate 1,4-beta-D-mannuronan block regions of alginate uncultured marine bacterium oligouronides
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 29000, SDS-PAGE uncultured marine bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 uncultured marine bacterium

pH Range

pH Minimum pH Maximum Comment Organism
5.8 9.1 less than 10% of maximal activity below pH 5.8 and above pH 9.1 uncultured marine bacterium