Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
gellan | Bacillus sp. (in: Bacteria) | degradation of gellan | ? | - |
? | |
gellan | Bacillus sp. (in: Bacteria) GL1 | degradation of gellan | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | O82833 | - |
- |
Bacillus sp. (in: Bacteria) GL1 | O82833 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | of the bacterium grown in the presence of gellan as a carbon source | Bacillus sp. (in: Bacteria) | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.34 | - |
pH 7.0, 30°C | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
gellan | degradation of gellan | Bacillus sp. (in: Bacteria) | ? | - |
? | |
gellan | degradation of gellan | Bacillus sp. (in: Bacteria) GL1 | ? | - |
? | |
[beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-]n | i.e. deacetylated gellan. The purified gellan lyase depolymerizes deacetylated gellan and gives a single oligosaccharide product with a molecular weight of 646 Da, which is determined by fast atom bombardment mass spectrometry. The structure of the product is determined by the combination of mass spectrometry, HPLC analysis, and high-resolution proton nuclear magnetic resonance spectroscopy to be a tetrasaccharide of glucuronyl-glucosyl-rhamnosyl-glucose with unsaturated glucuronic acid at the nonreducing terminal | Bacillus sp. (in: Bacteria) | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + [beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-]n-1 | - |
? | |
[beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-]n | i.e. deacetylated gellan. The purified gellan lyase depolymerizes deacetylated gellan and gives a single oligosaccharide product with a molecular weight of 646 Da, which is determined by fast atom bombardment mass spectrometry. The structure of the product is determined by the combination of mass spectrometry, HPLC analysis, and high-resolution proton nuclear magnetic resonance spectroscopy to be a tetrasaccharide of glucuronyl-glucosyl-rhamnosyl-glucose with unsaturated glucuronic acid at the nonreducing terminal | Bacillus sp. (in: Bacteria) GL1 | beta-D-4-deoxy-DELTA4,5-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + [beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-beta-D-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp-(1->4)-]n-1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 140000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Bacillus sp. (in: Bacteria) |