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Literature summary for 4.2.2.25 extracted from

  • Hashimoto, W.; Inose, T.; Nakajima, H.; Sato, N.; Kimura, S.; Murata, K.
    Purification and characterization of microbial gellan lyase (1996), Appl. Environ. Microbiol., 62, 1475-1477.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.25

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, 50% inhibition unidentified microorganism
Hg2+ 0.1 mM, significantly inhibits unidentified microorganism
additional information Ca2+, Mo2+, Cu2+, Fe2+, Co2+, and Zn2+ show no effects on enzyme activity at 1 mM. Dithiothreitol, glutathione (reduced form), and 2-mercaptoethanol (1 mM each) and N-ethylmaleimide (0.1 mM) exhibit no effect on enzyme activity unidentified microorganism

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 1 * 140000, SDS-PAGE unidentified microorganism

Organism

Organism UniProt Comment Textmining
unidentified microorganism
-
 soil microorganism, microbial strain for lyase production not specified
-

Purification (Commentary)

Purification (Comment) Organism
microbial strain for lyase production not specified unidentified microorganism

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 unidentified microorganism
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.671
-
 pH 7.0, 30°C unidentified microorganism

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gellan the enzyme is highly specific to gellan, especially deacetylated gellan, and other gellan-related polysaccharides, such as S-88, welan, rhamsan, and S-198. The viscosity of the gellan solution in the presence of the enzyme decreases and finally reaches a level comparable to that of water unidentified microorganism ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 140000, SDS-PAGE unidentified microorganism

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 unidentified microorganism

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 30°C: about 45% of maximal activity, 50°C: 50% of maximal activity unidentified microorganism

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 pH 7.5, stable below unidentified microorganism
50
-
 pH 7.5, 10 min, 50% loss of activity unidentified microorganism

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 in sodium N-2-hydroxyethylpiperazine-N'-2-ethanesulfonate (HEPES) buffer unidentified microorganism

pH Range

pH Minimum pH Maximum Comment Organism
5 10 pH 5.0: about 40% of maximal activity, pH 10.0: about 50% of maximal activity unidentified microorganism

Expression

Organism Comment Expression
unidentified microorganism indicible in presence of gellan up