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Literature summary for 4.2.2.24 extracted from

  • Ochiai, A.; Itoh, T.; Kawamata, A.; Hashimoto, W.; Murata, K.
    Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization (2007), Appl. Environ. Microbiol., 73, 3803-3813.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
D-glucose 5 mM, 1.2fold activation Bacillus subtilis
D-glucuronic acid 5 mM, 1.25fold activation Bacillus subtilis
D-sucrose 5 mM, 1.2fold activation Bacillus subtilis
D-xylose 5 mM, 1.37fold activation Bacillus subtilis
L-rhamnose 5 mM, 1.24fold activation Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Bacillus subtilis strain natto Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
D-galacturonic acid 5 mM, 41% loss of activity Bacillus subtilis
EDTA 10 mM, complete loss of activity Bacillus subtilis
EGTA 10 mM, complete loss of activity Bacillus subtilis
iodoacetic acid 1 mM, 45% loss of activity Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
rhamnogalacturonan I oligosaccharide Km-value is 1.9 mg/ml, pH 7.5, 30°C Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus subtilis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, addition the chelator-treated enzyme partly restores activity Bacillus subtilis
Co2+ 1 mM, addition the chelator-treated enzyme restores activity Bacillus subtilis
Mn2+ 1 mM, addition the chelator-treated enzyme restores activity Bacillus subtilis
Zn2+ 1 mM, addition the chelator-treated enzyme restores activity Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
1 * 68000, SDS-PAGE Bacillus subtilis
70000
-
gel filtration Bacillus subtilis
687544
-
1 * 687544, mature form of YesX including the C-terminal 8His-tag Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rhamnogalacturonan I oligosaccharide Bacillus subtilis the enzyme is part of the degradation system of rhamnogalacturonan type I. YesW catalyzes the initial cleavage of the rhamnogalacturonan I main chain, and the resultant oligosaccharides are converted to disaccharides through the extracellular exotype YesX reaction. The disaccharide is finally degraded into its constituent monosaccharides through the reaction of intracellular unsaturated galacturonyl hydrolases YesR and YteR 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end
-
?
rhamnogalacturonan I oligosaccharide Bacillus subtilis 168 the enzyme is part of the degradation system of rhamnogalacturonan type I. YesW catalyzes the initial cleavage of the rhamnogalacturonan I main chain, and the resultant oligosaccharides are converted to disaccharides through the extracellular exotype YesX reaction. The disaccharide is finally degraded into its constituent monosaccharides through the reaction of intracellular unsaturated galacturonyl hydrolases YesR and YteR 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O31527
-
-
Bacillus subtilis 168 O31527
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli cells Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
rhamnogalacturonan I oligosaccharide the enzyme is part of the degradation system of rhamnogalacturonan type I. YesW catalyzes the initial cleavage of the rhamnogalacturonan I main chain, and the resultant oligosaccharides are converted to disaccharides through the extracellular exotype YesX reaction. The disaccharide is finally degraded into its constituent monosaccharides through the reaction of intracellular unsaturated galacturonyl hydrolases YesR and YteR Bacillus subtilis 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end
-
?
rhamnogalacturonan I oligosaccharide rhamnogalacturonan I from potatoes. YesX acts on the substrate exolytically and releases disaccharides as major product. The enzyme mainly acts on rhamnogalacturonan I backbone, slight activity on polygalacturonan Bacillus subtilis 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end
-
?
rhamnogalacturonan I oligosaccharide the enzyme is part of the degradation system of rhamnogalacturonan type I. YesW catalyzes the initial cleavage of the rhamnogalacturonan I main chain, and the resultant oligosaccharides are converted to disaccharides through the extracellular exotype YesX reaction. The disaccharide is finally degraded into its constituent monosaccharides through the reaction of intracellular unsaturated galacturonyl hydrolases YesR and YteR Bacillus subtilis 168 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end
-
?
rhamnogalacturonan I oligosaccharide rhamnogalacturonan I from potatoes. YesX acts on the substrate exolytically and releases disaccharides as major product. The enzyme mainly acts on rhamnogalacturonan I backbone, slight activity on polygalacturonan Bacillus subtilis 168 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end
-
?

Subunits

Subunits Comment Organism
monomer 1 * 68000, SDS-PAGE Bacillus subtilis
monomer 1 * 687544, mature form of YesX including the C-terminal 8His-tag Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Bacillus subtilis
60
-
-
Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 75 40°C: about 40% of maximal activity, 75°C: about 50% of maximal activity Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
5 min, stable below Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10 pH 7.5: about 40% of maximal activity, pH 10.0: about 40% of maximal activity Bacillus subtilis