Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-glucose | 5 mM, 1.2fold activation | Bacillus subtilis | |
D-glucuronic acid | 5 mM, 1.25fold activation | Bacillus subtilis | |
D-sucrose | 5 mM, 1.2fold activation | Bacillus subtilis | |
D-xylose | 5 mM, 1.37fold activation | Bacillus subtilis | |
L-rhamnose | 5 mM, 1.24fold activation | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and Bacillus subtilis strain natto | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-galacturonic acid | 5 mM, 41% loss of activity | Bacillus subtilis | |
EDTA | 10 mM, complete loss of activity | Bacillus subtilis | |
EGTA | 10 mM, complete loss of activity | Bacillus subtilis | |
iodoacetic acid | 1 mM, 45% loss of activity | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
rhamnogalacturonan I oligosaccharide | Km-value is 1.9 mg/ml, pH 7.5, 30°C | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, addition the chelator-treated enzyme partly restores activity | Bacillus subtilis | |
Co2+ | 1 mM, addition the chelator-treated enzyme restores activity | Bacillus subtilis | |
Mn2+ | 1 mM, addition the chelator-treated enzyme restores activity | Bacillus subtilis | |
Zn2+ | 1 mM, addition the chelator-treated enzyme restores activity | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
1 * 68000, SDS-PAGE | Bacillus subtilis |
70000 | - |
gel filtration | Bacillus subtilis |
687544 | - |
1 * 687544, mature form of YesX including the C-terminal 8His-tag | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
rhamnogalacturonan I oligosaccharide | Bacillus subtilis | the enzyme is part of the degradation system of rhamnogalacturonan type I. YesW catalyzes the initial cleavage of the rhamnogalacturonan I main chain, and the resultant oligosaccharides are converted to disaccharides through the extracellular exotype YesX reaction. The disaccharide is finally degraded into its constituent monosaccharides through the reaction of intracellular unsaturated galacturonyl hydrolases YesR and YteR | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end | - |
? | |
rhamnogalacturonan I oligosaccharide | Bacillus subtilis 168 | the enzyme is part of the degradation system of rhamnogalacturonan type I. YesW catalyzes the initial cleavage of the rhamnogalacturonan I main chain, and the resultant oligosaccharides are converted to disaccharides through the extracellular exotype YesX reaction. The disaccharide is finally degraded into its constituent monosaccharides through the reaction of intracellular unsaturated galacturonyl hydrolases YesR and YteR | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O31527 | - |
- |
Bacillus subtilis 168 | O31527 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli cells | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
rhamnogalacturonan I oligosaccharide | the enzyme is part of the degradation system of rhamnogalacturonan type I. YesW catalyzes the initial cleavage of the rhamnogalacturonan I main chain, and the resultant oligosaccharides are converted to disaccharides through the extracellular exotype YesX reaction. The disaccharide is finally degraded into its constituent monosaccharides through the reaction of intracellular unsaturated galacturonyl hydrolases YesR and YteR | Bacillus subtilis | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end | - |
? | |
rhamnogalacturonan I oligosaccharide | rhamnogalacturonan I from potatoes. YesX acts on the substrate exolytically and releases disaccharides as major product. The enzyme mainly acts on rhamnogalacturonan I backbone, slight activity on polygalacturonan | Bacillus subtilis | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end | - |
? | |
rhamnogalacturonan I oligosaccharide | the enzyme is part of the degradation system of rhamnogalacturonan type I. YesW catalyzes the initial cleavage of the rhamnogalacturonan I main chain, and the resultant oligosaccharides are converted to disaccharides through the extracellular exotype YesX reaction. The disaccharide is finally degraded into its constituent monosaccharides through the reaction of intracellular unsaturated galacturonyl hydrolases YesR and YteR | Bacillus subtilis 168 | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end | - |
? | |
rhamnogalacturonan I oligosaccharide | rhamnogalacturonan I from potatoes. YesX acts on the substrate exolytically and releases disaccharides as major product. The enzyme mainly acts on rhamnogalacturonan I backbone, slight activity on polygalacturonan | Bacillus subtilis 168 | 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + shortened rhamnogalacturonan oligosaccharide containing one 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 68000, SDS-PAGE | Bacillus subtilis |
monomer | 1 * 687544, mature form of YesX including the C-terminal 8His-tag | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bacillus subtilis |
60 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 75 | 40°C: about 40% of maximal activity, 75°C: about 50% of maximal activity | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
5 min, stable below | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | pH 7.5: about 40% of maximal activity, pH 10.0: about 40% of maximal activity | Bacillus subtilis |