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Literature summary for 4.2.2.23 extracted from

  • Iwai, M.; Yamada, H.; Ikemoto, T.; Matsumoto, S.; Fujiwara, D.; Takenaka, S.; Sakamoto, T.
    Biochemical characterization and overexpression of an endo-rhamnogalacturonan lyase from Penicillium chrysogenum (2015), Mol. Biotechnol., 57, 539-548 .
    View publication on PubMed
Search for more literature for 4.2.2.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Penicillium chrysogenum

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 10% of initial activity Penicillium chrysogenum
Hg2+ 3% of initial activity Penicillium chrysogenum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 degalactosylated rhamnogalacturonan Km value 0.74 mg/ml, pH 7.0, 37°C Penicillium chrysogenum
additional information
-
 rhamnogalacturonan Km value 1.67 mg/ml, pH 7.0, 37°C Penicillium chrysogenum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Penicillium chrysogenum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 130% of initial activity Penicillium chrysogenum

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum W8VJU1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein protein does not contain N-linked carbohydrates Penicillium chrysogenum

Purification (Commentary)

Purification (Comment) Organism
from culture liquid Penicillium chrysogenum

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Penicillium chrysogenum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2 2.5 substrate rhamnogalacturonan, pH 7.0, 37°C Penicillium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azurine-crosslinked rhamnogalacturonan
-
 Penicillium chrysogenum azurine-crosslinked unsaturated rhamnogalacturonan oligosaccharides
-
 ?
degalactosylated rhamnogalacturonan
-
 Penicillium chrysogenum ?
-
 ?
additional information no substrate: homogalacturonan Penicillium chrysogenum ?
-
 ?
rhamnogalacturonan i.e. soybean rhamnogalacturonan Penicillium chrysogenum rhamnogalacturonan tetrasaccharide with 4-deoxy-4,5-unsaturated galacturonic acid at the nonreducing end
-
 ?

Subunits

Subunits Comment Organism
? x * 51000, SDS-PAGE, x * 54449, calculated from sequence Penicillium chrysogenum

Synonyms

Synonyms Comment Organism
Rgl4A
-
 Penicillium chrysogenum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Penicillium chrysogenum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 7.0, stable for at least 1 h Penicillium chrysogenum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Penicillium chrysogenum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 40°C, stable for at least 1 h Penicillium chrysogenum