Application | Comment | Organism |
---|---|---|
food industry | rhamnogalacturonan lyase is useful in the processing of fruit, where it is important that the commercial pectolytic enzyme preparations solubilize and hydrolyze the branched RG structures, which otherwise remain as colloidally dissolved polymers in the juice and lead to problems during filtration and clarification | Aspergillus aculeatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
rhamnogalacturonan I | KM-values of recombinant rhamnogalacturonan lyase towards enzyme-treated saponified modified hairy regions of pectin | Aspergillus aculeatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
gel filtration | Aspergillus aculeatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus aculeatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native and recombinant enzyme | Aspergillus aculeatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Aspergillus aculeatus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
rhamnogalacturonan I | rhamnogalacturonan I domains in saponified hairy regions of apple pectin. The enzyme fragments rhamnogalacturonan I by a multiple attack mechanism. The catalytic efficiency of recombinant rhamnogalacturonan lyase increases with decreasing degree of acetylation. Removal of arabinose side chains improves the action of recombinant rhamnogalacturonan lyase. Removal of galactose side chains decreases the catalytic efficiency of recombinant rhamnogalacturonanlyase. The average degree of multiple attack is 2.5 (the degree of multiple attack is defined as the average number of catalytic events, following the first, during the lifetime of an individual enzyme-substrate complex) | Aspergillus aculeatus | rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end | major oligomeric reaction products contain an alternating rhamnogalacturonan backbone with a degree of polymerization of 4, 6, 8, and 10 and with an alpha-D-(4,5)-unsaturated D-galactopyranosyluronic acid at the nonreducing end and an L-rhamnopyranose at the reducing end | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 57000, SDS-PAGE | Aspergillus aculeatus |
Synonyms | Comment | Organism |
---|---|---|
RG-lyase | - |
Aspergillus aculeatus |
rhamnogalacturonan alpha-L-rhamnopyranosyl-(1,4)-alpha-D-galactopyranosyluronide lyase | - |
Aspergillus aculeatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | - |
Aspergillus aculeatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
pH 5.0, 4.5 h, stable | Aspergillus aculeatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Aspergillus aculeatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 40°C, stable | Aspergillus aculeatus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus aculeatus | zymography | 5.3 | 5.1 |