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Literature summary for 4.2.2.23 extracted from

  • Ochiai, A.; Itoh, T.; Mikami, B.; Hashimoto, W.; Murata, K.
    Structural determinants responsible for substrate recognition and mode of action in family 11 polysaccharide lyases (2009), J. Biol. Chem., 284, 10181-10189.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization of YesW in complex with rhamnose, sitting drop vapor diffusion method, structure determined at 1.32 A and 1.65 resolution, respectively Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
rhamnogalacturonan I KM-value is 0.181 mg/ml, pH 7.5, 30°C Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus subtilis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64444
-
x * 64444, calculated from sequence Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rhamnogalacturonan I Bacillus subtilis responsible for an initial cleavage of the rhamnogalacturonan I region of plant cell wall pectin. Bacillus subtilis strain 168 secretes two rhamnogalacturonan lyases, YesW and YesX, extracellularly. YesW cleaves the glycoside bond of the rhamnogalacturonan chain endolytically, and the resultant oligosaccharides are subsequently converted to disaccharides, unsaturated galacturonyl rhamnose, through the exotype YesX reaction rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O31526
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
rhamnogalacturonan I responsible for an initial cleavage of the rhamnogalacturonan I region of plant cell wall pectin. Bacillus subtilis strain 168 secretes two rhamnogalacturonan lyases, YesW and YesX, extracellularly. YesW cleaves the glycoside bond of the rhamnogalacturonan chain endolytically, and the resultant oligosaccharides are subsequently converted to disaccharides, unsaturated galacturonyl rhamnose, through the exotype YesX reaction Bacillus subtilis rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end
-
?
rhamnogalacturonan I endotype eliminative cleavage, the YesW amino acid residues Asn152, Asp172, Asn532, Gly533, Thr534, and Tyr595 in the active cleft bind to rhamnose molecules through hydrogen bonds and van der Waals contacts Bacillus subtilis rhamnogalacturonan I oligosaccharides with alpha-L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end YesW releases a tetrasaccharide and larger saccharides as a major product ?

Subunits

Subunits Comment Organism
? x * 64444, calculated from sequence Bacillus subtilis

Synonyms

Synonyms Comment Organism
YesW
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Bacillus subtilis