Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | activity in 20 mM phosphate buffer is less than half of the activity in 50 mM phosphate buffer, maximum activity for isoenzyme I in 50 mM, isoenzyme II in 50-100 mM phosphate | Bacteroides thetaiotaomicron |
General Stability | Organism |
---|---|
sensitive to lyophilization and freezing and thawing, both isoenzyme I and II | Bacteroides thetaiotaomicron |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
heparin | 50% inhibition at 0.050 mg/ml for enzyme I and 0.005 mg/ml for enzyme II | Bacteroides thetaiotaomicron | |
N-desulfated heparin | 50% inhibition at 0.150 mg/ml for enzyme I and 0.025 mg/ml for enzyme II | Bacteroides thetaiotaomicron |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
104000 | - |
x * 108000, isoenzyme I, x * 104000, isoenzyme II, SDS-PAGE | Bacteroides thetaiotaomicron |
108000 | - |
x * 108000, isoenzyme I, x * 104000, isoenzyme II, SDS-PAGE | Bacteroides thetaiotaomicron |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides thetaiotaomicron | - |
isoenzymes I and II | - |
Storage Stability | Organism |
---|---|
4°C, two weeks, 70-80% residual activity, both isoenzyme I and II | Bacteroides thetaiotaomicron |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chondroitin sulfate A | - |
Bacteroides thetaiotaomicron | ? | - |
? | |
chondroitin sulfate C | - |
Bacteroides thetaiotaomicron | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 108000, isoenzyme I, x * 104000, isoenzyme II, SDS-PAGE | Bacteroides thetaiotaomicron |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
isoenzyme I | Bacteroides thetaiotaomicron |
7.6 | - |
isoenzyme II | Bacteroides thetaiotaomicron |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacteroides thetaiotaomicron | isoelectric focusing, isoenzyme I | - |
7.9 |
Bacteroides thetaiotaomicron | isoelectric focusing, isoenzyme II | - |
8 |