Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.2.21 extracted from

  • Linn, S.; Chan, T.; Lipeski, L.; Salyers, A.A.
    Isolation and characterization of two chondroitin lyases from Bacteroides thetaiotaomicron (1983), J. Bacteriol., 156, 859-866.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
phosphate activity in 20 mM phosphate buffer is less than half of the activity in 50 mM phosphate buffer, maximum activity for isoenzyme I in 50 mM, isoenzyme II in 50-100 mM phosphate Bacteroides thetaiotaomicron

General Stability

General Stability Organism
sensitive to lyophilization and freezing and thawing, both isoenzyme I and II Bacteroides thetaiotaomicron

Inhibitors

Inhibitors Comment Organism Structure
heparin 50% inhibition at 0.050 mg/ml for enzyme I and 0.005 mg/ml for enzyme II Bacteroides thetaiotaomicron
N-desulfated heparin 50% inhibition at 0.150 mg/ml for enzyme I and 0.025 mg/ml for enzyme II Bacteroides thetaiotaomicron

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
104000
-
x * 108000, isoenzyme I, x * 104000, isoenzyme II, SDS-PAGE Bacteroides thetaiotaomicron
108000
-
x * 108000, isoenzyme I, x * 104000, isoenzyme II, SDS-PAGE Bacteroides thetaiotaomicron

Organism

Organism UniProt Comment Textmining
Bacteroides thetaiotaomicron
-
isoenzymes I and II
-

Storage Stability

Storage Stability Organism
4°C, two weeks, 70-80% residual activity, both isoenzyme I and II Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin sulfate A
-
Bacteroides thetaiotaomicron ?
-
?
chondroitin sulfate C
-
Bacteroides thetaiotaomicron ?
-
?

Subunits

Subunits Comment Organism
? x * 108000, isoenzyme I, x * 104000, isoenzyme II, SDS-PAGE Bacteroides thetaiotaomicron

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
isoenzyme I Bacteroides thetaiotaomicron
7.6
-
isoenzyme II Bacteroides thetaiotaomicron

pI Value

Organism Comment pI Value Maximum pI Value
Bacteroides thetaiotaomicron isoelectric focusing, isoenzyme I
-
7.9
Bacteroides thetaiotaomicron isoelectric focusing, isoenzyme II
-
8