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Literature summary for 4.2.2.20 extracted from

  • Hamai, A.; Hashimoto, N.; Mochizuki, H.; Kato, F.; Makiguchi, Y.; Horie, K.; Suzuki, S.
    Two distinct chondroitin sulfate ABC lyases. An endoeliminase yielding tetrasaccharides and an exoeliminase preferentially acting on oligosaccharides (1997), J. Biol. Chem., 272, 9123-9130.
    View publication on PubMed
Search for more literature for 4.2.2.20

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 80% inhibition Proteus vulgaris
Fe2+ 1 mM, 80% inhibition Proteus vulgaris
additional information not inhibitory: Ca2+, Mg2+, Ba2+, Mn2+ Proteus vulgaris
Ni2+ 1 mM, 80% inhibition Proteus vulgaris
Zn2+ 1 mM, complete inhibition Proteus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.066
-
 chondroitin-6-sulfate pH 8.0, 37°C Proteus vulgaris

Organism

Organism UniProt Comment Textmining
Proteus vulgaris
-
 NCTC 4636
-
Proteus vulgaris
-
 induced by chondroitin sulfate, isoenzyme ChS ABC lyase II
-

Purification (Commentary)

Purification (Comment) Organism
-
 Proteus vulgaris

Reaction

Reaction Comment Organism Reaction ID
R1-[beta-D-GalNAc-1,4-L-iduronic acid]-R2 = R1-[beta-D-GalNAc] + [4-deoxy-L-idu-4-enuronic acid]-R2 related enzyme EC 4.2.2.21 Proteus vulgaris
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
310
-
 pH 8.0, 37°C Proteus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin 32% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
chondroitin 4-sulfate 121% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
chondroitin 6-sulfate
-
 Proteus vulgaris unsaturated chondroitin 6-sulfate disaccharide
-
 ?
chondroitin sulfate D 70% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
chondroitin sulfate E 23% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
chondroitin sulfate proteoglycan 70% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
dermatan sulfate 55% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
hyaluronan 1% of the rate with chondroitin-6-sulfate Proteus vulgaris ?
-
 ?
additional information no substrate: keratan sulfate, heparin, heparan sulfate. Enzyme produces a wide range of differently sized oligosaccharides. After exhaustive degradation, end products are tetra- and disaccharides Proteus vulgaris ?
-
 ?

Synonyms

Synonyms Comment Organism
ChS ABC lyase I
-
 Proteus vulgaris
EC 4.2.2.4 formerly Proteus vulgaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Proteus vulgaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 30 min, 47% loss of activity Proteus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Proteus vulgaris

pI Value

Organism Comment pI Value Maximum pI Value
Proteus vulgaris and 8.25, isoelectric focusing
-
 8
Proteus vulgaris and 8.0, isoelectric focusing
-
 8.25