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Literature summary for 4.2.2.2 extracted from
- Improving the specific activity and thermo-stability of alkaline pectate lyase from Bacillus subtilis 168 for bioscouring (2018), Biochem. Eng. J., 129, 74-83 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus subtilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K47D/V132F | 2.2fold improvement in specific activity compared to wild-type | Bacillus subtilis |
| K47D/V132F/R272W | specific activity is significantly improved by about 400% in the presence of 1 mM Ca2+. Half-life at 50°C is extended to 330 min. Mutant can significantly improve the wettability and softness of fabrics | Bacillus subtilis |
| K47E | displays 1.8fold increase in activity, and half-life increased by 2.0fold at 50°C | Bacillus subtilis |
| K47E/V132F | 3.9fold improvement in specific activity compared to wild-type | Bacillus subtilis |
| V132F | mutant shows 1.7fold increase in activity with wider pH stability | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Polygalacturonate | Km value of wild-type 0.47 mg/ml, mutant V132F, 0.30 mg/ml, mutant K47E, 0.31 mg/ml, mutant K47E/V132F, 0.30 mg/ml, mutant K47D/V132F, 0.30 mg/ml, mutant K47D/V132F/R272W, 0.29 mg/ml, respectively, pH 9.5, 55°C | Bacillus subtilis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activity of wild-type peaks at 1 mM Ca2+, and declines with the increase in Ca2+concentration | Bacillus subtilis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | P39116 | - |
- |
| Bacillus subtilis 168 | P39116 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 353.6 | - |
wild-type, pH 9.5, 55°C | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonate | - |
Bacillus subtilis | unsaturated oligogalacturonides | - |
? | |
| polygalacturonate | - |
Bacillus subtilis 168 | unsaturated oligogalacturonides | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 241 | - |
Polygalacturonate | wild-type, pH 9.5, 55°C | Bacillus subtilis | |
| 354 | - |
Polygalacturonate | mutant V132F, pH 9.5, 55°C | Bacillus subtilis | |
| 356 | - |
Polygalacturonate | mutant K47E, pH 9.5, 55°C | Bacillus subtilis | |
| 365 | - |
Polygalacturonate | mutant K47E/V132F, pH 9.5, 55°C | Bacillus subtilis | |
| 390 | - |
Polygalacturonate | mutant K47D/V132F, pH 9.5, 55°C | Bacillus subtilis | |
| 461 | - |
Polygalacturonate | mutant K47D/V132F/R272W, pH 9.5, 55°C | Bacillus subtilis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Polygalacturonate | kcat/Km value of wild-type 513 ml/mg/s, mutant V132F, 1179 ml/mg/s, mutant K47E, 1144 ml/mg/s, mutant K47E/V132F, 1216 ml/mg/s, mutant K47D/V132F, 1318 ml/mg/s, mutant K47D/V132F/R272W, 1568 ml/mg/s, respectively, pH 9.5, 55°C | Bacillus subtilis | |
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