Literature summary for 4.2.2.2 extracted from

Liang, C.; Gui, X.; Zhou, C.; Xue, Y.; Ma, Y.; Tang, S.Y.
Improving the thermoactivity and thermostability of pectate lyase from Bacillus pumilus for ramie degumming (2015), Appl. Microbiol. Biotechnol., 99, 2673-2682 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bacillus pumilus

Protein Variants

Protein Variants	Comment	Organism
R227W	increase in expression and specific activity, no increase in thermostability	Bacillus pumilus
R227W/S145C/T206A	increase in specific activity and thermostability	Bacillus pumilus
R227W/S145C/T50T	increase in specific activity and thermostability	Bacillus pumilus
R227W/T206A/T222T	increase in specific activity and thermostability	Bacillus pumilus

Organism

Organism	UniProt	Comment	Textmining
Bacillus pumilus	EDW21517	-	-
Bacillus pumilus ATCC 7061	EDW21517	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
130.9	-	wild-type, pH 10, 60°C	Bacillus pumilus
144.4	-	mutant R227W, pH 10, 60°C	Bacillus pumilus
185.9	-	mutant R227W/S145C/T50T, pH 10, 60°C	Bacillus pumilus
326.8	-	mutant R227W/T206A/T222T, pH 10, 60°C	Bacillus pumilus
438.7	-	mutant R227W/S145C/T206A, pH 10, 60°C	Bacillus pumilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
polygalacturonate	-	Bacillus pumilus	unsaturated oligogalacturonides	-	?
polygalacturonate	-	Bacillus pumilus ATCC 7061	unsaturated oligogalacturonides	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	half-live of inactivation is 2.7 min for wild-type, 3.1 min for mutant R227W, 6.9 min for mutant R227W/S145C/T50T, 190.5 min for mutant R227W/T206A/T222T, 800.3 min for mutant R227W/S145C/T206A	Bacillus pumilus

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Release 2023.1 (January 2023)