Cloned (Comment) | Organism |
---|---|
- |
Paenibacillus sp. 0602 |
Crystallization (Comment) | Organism |
---|---|
structure of PelN, to 1.45 A resolution | Paenibacillus sp. 0602 |
Protein Variants | Comment | Organism |
---|---|---|
D174A | mutation in Ca2+ binding site, complete loss of activity | Paenibacillus sp. 0602 |
K93I | 1.75fold increase in specific activity, folding free energy decreases by 1.03 kcal/ml | Paenibacillus sp. 0602 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | residues D152, D174, and D178 form the Ca2+ ion binding sites | Paenibacillus sp. 0602 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. 0602 | W8CR80 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1602 | - |
wild-type, pH 9.8, 50°C | Paenibacillus sp. 0602 |
2797 | - |
mutant K93I, pH 9.8, 50°C | Paenibacillus sp. 0602 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
polygalacturonate | - |
Paenibacillus sp. 0602 | ? | - |
? | |
ramie fiber | - |
Paenibacillus sp. 0602 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PelN | - |
Paenibacillus sp. 0602 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
67.5 | - |
wild-type | Paenibacillus sp. 0602 |