Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.2.2 extracted from

  • Yuan, P.; Meng, K.; Shi, P.; Luo, H.; Huang, H.; Tu, T.; Yang, P.; Yao, B.
    An alkaline-active and alkali-stable pectate lyase from Streptomyces sp. S27 with potential in textile industry (2012), J. Ind. Microbiol. Biotechnol., 39, 909-915.
    View publication on PubMed
Search for more literature for 4.2.2.2

Cloned(Commentary)

Cloned (Comment) Organism
gene pl-str, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3), subcloning in Escherichia coli strain JM109 Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 8% activation at 1 mM, 78.5% inhibition at 5 mM Streptomyces sp.
Cr3+ inhibits completely at 1 mM Streptomyces sp.
Cu2+ 11% activation at 1 mM, 41% inhibition at 5 mM Streptomyces sp.
EDTA inhibits completely at 1 mM Streptomyces sp.
Fe3+ 4% inhibition at 1 mM, 99% inhibition at 5 mM Streptomyces sp.
Li+ 5% activation at 1 mM, 62% inhibition at 5 mM Streptomyces sp.
Mg2+ 9% activation at 1 mM, 22% inhibition at 5 mM Streptomyces sp.
Ni2+ 4% inhibition at 1 mM, 93% inhibition at 5 mM Streptomyces sp.
Pb2+ 31% inhibition at 1 mM, complete inhibition at 5 mM Streptomyces sp.
Zn2+ 13% activation at 1 mM, 46% inhibition at 5 mM Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required, optimal at about 0.05 mM Streptomyces sp.
K+ 9% activation at 1 mM Streptomyces sp.
Mn2+ 29% activation at 1 mM, 7.5% activation at 5 mM Streptomyces sp.
Na+ 10% activation at 1 mM Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33500
-
 x * 33500, about, sequence calculation, x * 35000, recombinant enzyme, SDS-PAGE Streptomyces sp.
35000
-
 x * 33500, about, sequence calculation, x * 35000, recombinant enzyme, SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
 isolated from soil of Flaming Mountain in the Turpan Basin of Xinjiang, China, gene pl-str
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatgraphy Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citrus pectin P9311
-
 Streptomyces sp. ?
-
 ?
citrus pectin P9436
-
 Streptomyces sp. ?
-
 ?
citrus pectin P9561
-
 Streptomyces sp. ?
-
 ?
polygalacturonic acid
-
 Streptomyces sp. ?
-
 ?

Subunits

Subunits Comment Organism
? x * 33500, about, sequence calculation, x * 35000, recombinant enzyme, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
PL-STR
-
 Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 purified recombinant His-tagged enzyme, 1 h, without substrate, pH 12.0, over 58% activity remaining Streptomyces sp.
37
-
 purified recombinant His-tagged enzyme, 1 h, without substrate, pH 7.0-10.0, over 80% activity remaining Streptomyces sp.
37 50 purified recombinant His-tagged enzyme, 45 min, without substrate, pH 9.0-12.0, over 80% activity remaining Streptomyces sp.
50
-
 purified recombinant His-tagged enzyme, 45 min, without substrate, pH 10.0, over 80% activity remaining Streptomyces sp.
60
-
 purified recombinant His-tagged enzyme, 45 min, without substrate, pH 10.0, 30% activity remaining Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10 purified recombinant His-tagged enzyme, 1 h, 60°C, without substrate, 30% activity remaining, at 37-50°C over 80% activity remain Streptomyces sp.
9 12 purified recombinant His-tagged enzyme, 1 h, 60°C, without substrate, 30% activity remaining, at 37-50°C over 80% activity remain Streptomyces sp.

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces sp. sequence calculation
-
 6.83

General Information

General Information Comment Organism
evolution PL-STR belongs to family PF00544 Streptomyces sp.