Literature summary for 4.2.2.2 extracted from

Zheng, Y.; Huang, C.H.; Liu, W.; Ko, T.P.; Xue, Y.; Zhou, C.; Guo, R.T.; Ma, Y.
Crystal structure and substrate-binding mode of a novel pectate lyase from alkaliphilic Bacillus sp. N16-5 (2012), Biochem. Biophys. Res. Commun., 420, 269-274.

Application

Application	Comment	Organism
industry	pectate lyase (Bsp165PelA) from Bacillus sp. N16-5 has a great potential in industrial applications because it shows high specific activity under extremely alkaline conditions	Bacillus sp. (in: Bacteria)

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme, alone or complexed with trigalacturonate, sitting drop method, 0.002 ml of 12.5 mg/ml protein in 25 mM Tris-HCl, 150 mM NaCl, pH 8.0, are mixed with 0.002 ml of 0.18 M Li2SO4, 0.085 M Tris-HCl, pH 8.5, 24% PEG 4000, and 15% v/v glycerol anhydrous, and equilibrated against 0.5 ml of well solution, three days, X-ray diffraction structure determination and analysis at 1.5-1.9 A resolution	Bacillus sp. (in: Bacteria)

Crystallization (Comment)

Organism

purified enzyme, alone or complexed with trigalacturonate, sitting drop method, 0.002 ml of 12.5 mg/ml protein in 25 mM Tris-HCl, 150 mM NaCl, pH 8.0, are mixed with 0.002 ml of 0.18 M Li2SO4, 0.085 M Tris-HCl, pH 8.5, 24% PEG 4000, and 15% v/v glycerol anhydrous, and equilibrated against 0.5 ml of well solution, three days, X-ray diffraction structure determination and analysis at 1.5-1.9 A resolution

Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	dependent on, binding structure, overview	Bacillus sp. (in: Bacteria)

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. (in: Bacteria)	-	-	-
Bacillus sp. (in: Bacteria) N16-5	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
trigalacturonate	enzyme and substrate-binding structures, overview	Bacillus sp. (in: Bacteria)	?	-	?
trigalacturonate	enzyme and substrate-binding structures, overview	Bacillus sp. (in: Bacteria) N16-5	?	-	?

Synonyms

Synonyms	Comment	Organism
Bsp165PelA	-	Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
11.5	-	-	Bacillus sp. (in: Bacteria)

General Information

General Information	Comment	Organism
evolution	parallel beta-helix, active site residues, and substrate binding cleft are similar to those in the other pectate lyases from polysaccharide lyase family 1, PL1	Bacillus sp. (in: Bacteria)