Literature summary for 4.2.2.2 extracted from

Wang, H.; Fu, L.; Zhang, X.
Comparison of expression, purification and characterization of a new pectate lyase from Phytophthora capsici using two different methods (2011), BMC Biotechnol., 11, 32.

Cloned(Commentary)

Cloned (Comment)	Organism
gene Pcpel2, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli BL21(DE3) using the pMAL or pLysS system, expression of maltose binding fusion protein, MBP-PCPEL2	Phytophthora capsici

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant PCPEL2, hanging-drop vapour-diffusion method, 16°C, 0.001 ml of protein solution containing 5 mg/ml protein in 10 mM Tris-HCl, pH 7.5, 150 mM NaCl and 1 mM DTT, is mixed with 0.001 ml of reservoir solution containing 0.1 M HEPES, pH 7.5, 10% w/v PEG 6000, 5% v/v 2-methy-2,4-pentanediol, equilibration versus 0.2 ml of reservoir solution, 5 days, X-ray diffraction structure determination and analysis	Phytophthora capsici

Crystallization (Comment)

Organism

purified recombinant PCPEL2, hanging-drop vapour-diffusion method, 16°C, 0.001 ml of protein solution containing 5 mg/ml protein in 10 mM Tris-HCl, pH 7.5, 150 mM NaCl and 1 mM DTT, is mixed with 0.001 ml of reservoir solution containing 0.1 M HEPES, pH 7.5, 10% w/v PEG 6000, 5% v/v 2-methy-2,4-pentanediol, equilibration versus 0.2 ml of reservoir solution, 5 days, X-ray diffraction structure determination and analysis

Phytophthora capsici

Organism

Organism	UniProt	Comment	Textmining
Phytophthora capsici	G2XKV7	gene Pcpel2	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged PCPEL2 from Escherichia coli strain BL21(DE3), 34.1fold from the pLysS system and 53.8fold from the pMal system, by nickel affinity chromatography and gel filtration chromatography, recombinant MBP-PCPEL2 by amylose affinity chromatography	Phytophthora capsici

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
750	-	purified recombinant PCPEL2 expressed from pLysS, pH not specified in the publication, temperature not specified in the publication	Phytophthora capsici
860	-	purified recombinant PCPEL2 expressed from pMAL, pH not specified in the publication, temperature not specified in the publication	Phytophthora capsici

Synonyms

Synonyms	Comment	Organism
PCPEL2	-	Phytophthora capsici