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Literature summary for 4.2.2.2 extracted from
- Biochemical properties of pectate lyases produced by three different Bacillus strains isolated from fermenting cocoa beans and characterization of their cloned genes (2010), Appl. Environ. Microbiol., 76, 5214-5220.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determnination and analysis | Bacillus pumilus |
| DNA and amino acid sequence determnination and analysis | Bacillus subtilis |
| DNA and amino acid sequence determnination and analysis | Lysinibacillus fusiformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 38% inhibition at 0.2 mM | Bacillus subtilis |  |
| Ba2+ | 21% inhibition at 0.2 mM | Lysinibacillus fusiformis | |
| Mg2+ | 23% inhibition at 0.2 mM | Bacillus subtilis | |
| Mg2+ | 8.4% inhibition at 0.2 mM | Lysinibacillus fusiformis | |
| Mn2+ | 94% inhibition at 0.2 mM | Bacillus subtilis | |
| Mn2+ | 82% inhibition at 0.2 mM | Lysinibacillus fusiformis | |
| Ni2+ | 31% inhibition at 0.2 mM | Bacillus subtilis | |
| Se2+ | 18% inhibition at 0.2 mM | Bacillus subtilis | |
| Se2+ | 10% inhibition at 0.2 mM | Lysinibacillus fusiformis | |
| Zn2+ | 88% inhibition at 0.2 mM | Bacillus subtilis | |
| Zn2+ | 97% inhibition at 0.2 mM | Lysinibacillus fusiformis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | activates 181% at 0.2 mM | Bacillus pumilus | |
| Ca2+ | activates 208% at 0.2 mM | Bacillus pumilus | |
| Ca2+ | activates 208% at 0.2 mM | Lysinibacillus fusiformis | |
| Ca2+ | activates 263% at 0.2 mM | Bacillus subtilis | |
| Cu2+ | activates 19% at 0.2 mM | Lysinibacillus fusiformis | |
| Cu2+ | activates 193% at 0.2 mM | Bacillus pumilus | |
| Fe2+ | activates 213% at 0.2 mM | Bacillus pumilus | |
| Fe2+ | activates 231% at 0.2 mM | Bacillus pumilus | |
| Mg2+ | activates 190% at 0.2 mM | Bacillus pumilus | |
| Mn2+ | activates 136% at 0.2 mM | Bacillus pumilus | |
| additional information | Fe2+ and Cu2+ do not significantly affect the activity of Pel-66 | Bacillus subtilis | |
| additional information | Fe2+ and Ni2+ do not significantly affect the activity of Pel-90 | Lysinibacillus fusiformis | |
| Ni2+ | activates 191% at 0.2 mM | Bacillus pumilus | |
| Se2+ | activates 158% at 0.2 mM | Bacillus pumilus | |
| Zn2+ | activates 132% at 0.2 mM | Bacillus pumilus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus pumilus | D8X181 | isolated from fermenting cocoa beans | - |
| Bacillus pumilus BS22 | D8X181 | isolated from fermenting cocoa beans | - |
| Bacillus subtilis | D8X180 | isolated from fermenting cocoa beans | - |
| Bacillus subtilis BS66 | D8X180 | isolated from fermenting cocoa beans | - |
| Lysinibacillus fusiformis | D8X179 | Lysinibacillus fusiformis, isolated from fermenting cocoa beans | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native Pel-22 from supernatant by ammonium sulfate fractionation and cation exchange chromatography | Bacillus pumilus |
| native Pel-66 from supernatant by ammonium sulfate fractionation and cation exchange chromatography | Bacillus subtilis |
| native Pel-90 from supernatant by ammonium sulfate fractionation and cation exchange chromatography | Lysinibacillus fusiformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrates are 45% methylated pectin or polygalacturonate | Bacillus pumilus | ? | - |
? | |
| additional information | substrates are 45% methylated pectin or polygalacturonate | Bacillus subtilis | ? | - |
? | |
| additional information | substrates are 45% methylated pectin or polygalacturonate | Lysinibacillus fusiformis | ? | - |
? | |
| additional information | substrates are 45% methylated pectin or polygalacturonate | Bacillus subtilis BS66 | ? | - |
? | |
| additional information | substrates are 45% methylated pectin or polygalacturonate | Bacillus pumilus BS22 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pel-22 | - |
Bacillus pumilus |
| Pel-66 | - |
Bacillus subtilis |
| Pel-90 | - |
Lysinibacillus fusiformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bacillus pumilus |
| 37 | - |
assay at | Bacillus subtilis |
| 37 | - |
assay at | Lysinibacillus fusiformis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | activity range | Bacillus pumilus |
| 30 | 70 | activity range | Bacillus subtilis |
| 30 | 70 | activity range | Lysinibacillus fusiformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bacillus pumilus |
| 8 | - |
- |
Bacillus subtilis |
| 8 | - |
- |
Lysinibacillus fusiformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10.5 | activity range | Bacillus pumilus |
| 3 | 10.5 | activity range | Bacillus subtilis |
| 3 | 10.5 | activity range | Lysinibacillus fusiformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | pH ranging from 3.0 to 10.0 does not significantly influence the stability of the enzyme, but the enzyme seems to be more stable at its optimum pH | Bacillus pumilus |
| 3 | 10 | pH ranging from 3.0 to 10.0 does not significantly influence the stability of the enzyme, but the enzyme seems to be more stable at its optimum pH | Bacillus subtilis |
| 3 | 10 | pH ranging from 3.0 to 10.0 does not significantly influence the stability of the enzyme, but the enzyme seems to be more stable at its optimum pH | Lysinibacillus fusiformis |
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