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Literature summary for 4.2.2.2 extracted from

  • Li, P.; Jing, Q.; Shao, W.
    Expression, purification and characterization of a thermostable pectate lyase from Thermotoga maritima (2009), Sheng Wu Gong Cheng Xue Bao, 25, 223-229.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
Polygalacturonate
-
Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ PelC is dependent on Ca2+ for activity and stability Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
recombinant enzyme, SDS-PAGE Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polygalacturonate
-
Thermotoga maritima unsaturated oligogalacturonides
-
?
polygalacturonate
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 unsaturated oligogalacturonides
-
?

Synonyms

Synonyms Comment Organism
pectate lyase C
-
Thermotoga maritima
pelC
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 95 the enzyme keeps stable, possesses a high level of activity at 60°C and a half-life of almost 2 h at 95°C Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Thermotoga maritima

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.2 9.8
-
Thermotoga maritima