Any feedback?
Literature summary for 4.2.2.2 extracted from
- Cloning, expression, and characterization of a highly active alkaline pectate lyase from alkaliphilic Bacillus sp. N16-5 (2010), J. Microbiol. Biotechnol., 20, 670-677.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) and DH5alpha cells | Bacillus sp. (in: Bacteria) |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 25% residual activity at 1 mM | Bacillus sp. (in: Bacteria) |  |
| Ca2+ | Ca2+ does not increase but instead inhibits the activity of PelA, 41% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
| Co2+ | 22% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
| Cu2+ | 24% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
| EDTA | PelA is sensitive to 10 mM EDTA with 39% activity retained | Bacillus sp. (in: Bacteria) | |
| Hg2+ | 24% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
| Mg2+ | 56% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
| Mn2+ | complete inhibition at 1 mM | Bacillus sp. (in: Bacteria) | |
| Ni2+ | 67% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
| Pb2+ | 53% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
| SDS | PelA is sensitive to 0.1% (w/v) SDS with 43% activity retained | Bacillus sp. (in: Bacteria) | |
| Sn2+ | 75% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
| Zn2+ | 69% residual activity at 1 mM | Bacillus sp. (in: Bacteria) | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | Ca2+ is not required for activity on pectic substrates | Bacillus sp. (in: Bacteria) | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35940 | - |
calculated from amino acid sequence | Bacillus sp. (in: Bacteria) |
| 42000 | - |
SDS-PAGE | Bacillus sp. (in: Bacteria) |
| 45000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | D0VP31 | - |
- |
| Bacillus sp. (in: Bacteria) N16-5 | D0VP31 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose column chromatography | Bacillus sp. (in: Bacteria) |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1000 | - |
after 25.3fold purification | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citrus pectin | PelA shows 25% relative activity on citrus pectin compared to polygalacturonate | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| citrus pectin | PelA shows 25% relative activity on citrus pectin compared to polygalacturonate | Bacillus sp. (in: Bacteria) N16-5 | ? | - |
? | |
| additional information | PelA does not show any activity on mannan, CMC, xylan, glucan, or soluble starch | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| additional information | PelA does not show any activity on mannan, CMC, xylan, glucan, or soluble starch | Bacillus sp. (in: Bacteria) N16-5 | ? | - |
? | |
| polygalacturonate | the enzyme is specific toward alpha-1,4-galacturonic acid linkages of galactopolysaccharides | Bacillus sp. (in: Bacteria) | unsaturated 4,5-digalacturonate + unsaturated 4,5-trigalacturonate + ? | main products | ? | |
| polygalacturonate | the enzyme is specific toward alpha-1,4-galacturonic acid linkages of galactopolysaccharides | Bacillus sp. (in: Bacteria) N16-5 | unsaturated 4,5-digalacturonate + unsaturated 4,5-trigalacturonate + ? | main products | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PelA | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 65 | more than 60% of the maximum activity is detectable between 40 and 65°C | Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | PelA is stable during 30°C to 40°C and not very stable at 50°C (in 50 mM glycine-NaOH buffer, pH 10.0), about 60% activity is retained after 30 min incubation at 50°C and it almost completely loses activity when it is incubated at 60°C | Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 11.5 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 12 | PelA is stable at pH 9.0 and 10.0 and maintains more than 85% activity after incubation at 40°C for 1 h, about 50% activity is retained when it is incubated at pH 11.0 and it almost completely loses activity when it is incubated at pH 12.0 | Bacillus sp. (in: Bacteria) |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | pI-value around pH 6.0, isoelectric focusing | - |
6 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
