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Literature summary for 4.2.2.2 extracted from
- Thermodynamic characterization of a highly thermoactive extracellular pectate lyase from a new isolate Bacillus pumilus DKS1 (2008), Biores. Technol., 99, 8088-8094.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Bacillus pumilus |  |
| Ni2+ | - |
Bacillus pumilus | |
| Zn2+ | - |
Bacillus pumilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | values of Km (polygalacturonic acid) is 0.44 mg/ml | Bacillus pumilus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus pumilus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | enhances activity | Bacillus pumilus | |
| Mg2+ | slightly enhances enzyme activity | Bacillus pumilus | |
| Mn2+ | enhances activity | Bacillus pumilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
SDS-PAGE | Bacillus pumilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus pumilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| an extracellular pectate lyase is purified from the culture filtrate of a newly isolated Bacillus pumilus DKS1 grown in pectin containing medium using ion-exchange and gel filtration chromatography | Bacillus pumilus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonic acid | - |
Bacillus pumilus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pectate lyase | - |
Bacillus pumilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Bacillus pumilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
beyond 70°C, enzyme activity is severely inhibited. The half-life is 6.93 min at 70°C as compared to 31 min at 40°C | Bacillus pumilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Bacillus pumilus |
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