Literature summary for 4.2.2.2 extracted from

Wang, Y.; Wang, Z.; Du, G.; Hua, Z.; Liu, L.; Li, J.; Chen, J.
Enhancement of alkaline polygalacturonate lyase production in recombinant Pichia pastoris according to the ratio of methanol to cell concentration (2009), Biores. Technol., 100, 1343-1349.

Application

Application	Comment	Organism
biotechnology	enhancing PGL production by controlling the optimal ratio provides an alternative approach to enhance heterologous protein production with Pichia pastoris	Bacillus subtilis

Cloned(Commentary)

Cloned (Comment)	Organism
recombinantly expressed in Pichia pastoris. The ratio of methanol to cell concentration has a significant influence on PGL production. An advanced glycerol exponential feeding strategy is developed for biomass accumulation in cell growth phase, by which cell concentration reaches 140 g/L after 19 h glycerol feeding. In subsequent production phase, a methanol feeding profile is proposed according to the optimal ratio of methanol to cell concentration at a range of 0.163-0.171 g/g, and PGL activity and productivity reaches 430 U/ml and 4.34 U/ml/h, respectively	Bacillus subtilis

Cloned (Comment)

Organism

recombinantly expressed in Pichia pastoris. The ratio of methanol to cell concentration has a significant influence on PGL production. An advanced glycerol exponential feeding strategy is developed for biomass accumulation in cell growth phase, by which cell concentration reaches 140 g/L after 19 h glycerol feeding. In subsequent production phase, a methanol feeding profile is proposed according to the optimal ratio of methanol to cell concentration at a range of 0.163-0.171 g/g, and PGL activity and productivity reaches 430 U/ml and 4.34 U/ml/h, respectively

Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
polygalacturonate	-	Bacillus subtilis	unsaturated polygalacturonic acid	-	?

Synonyms

Synonyms	Comment	Organism
alkaline polygalacturonate lyase	-	Bacillus subtilis
PGL	-	Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
45	-	assay at	Bacillus subtilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9.4	-	assay at	Bacillus subtilis

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Release 2023.1 (January 2023)