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Literature summary for 4.2.2.2 extracted from
- A novel thermophilic pectate lyase containing two catalytic modules of Clostridium stercorarium (2005), Biosci. Biotechnol. Biochem., 69, 2138-2145.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Thermoclostridium stercorarium |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | strong activation, optimal activity at 0.05 mM CaCl2 | Thermoclostridium stercorarium |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 135171 | - |
x * 135171, calculated from sequence | Thermoclostridium stercorarium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoclostridium stercorarium | Q76EC9 | - |
- |
| Thermoclostridium stercorarium F-9 | Q76EC9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Thermoclostridium stercorarium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin | apple or citrus pectin, activity with pectin is lower than activity with polygalacturonate | Thermoclostridium stercorarium | ? | - |
? | |
| pectin | apple or citrus pectin, activity with pectin is lower than activity with polygalacturonate | Thermoclostridium stercorarium F-9 | ? | - |
? | |
| polygalacturonic acid | - |
Thermoclostridium stercorarium | ? | - |
? | |
| polygalacturonic acid | - |
Thermoclostridium stercorarium F-9 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 135171, calculated from sequence | Thermoclostridium stercorarium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pectate lyase 9A | - |
Thermoclostridium stercorarium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Thermoclostridium stercorarium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 70 | about 30% of maximal activity at 50°C and at 70°C | Thermoclostridium stercorarium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
10 min, stable up to | Thermoclostridium stercorarium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Thermoclostridium stercorarium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | 4°C, 12 h, stable in the range | Thermoclostridium stercorarium |
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Release 2023.1 (January 2023)
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