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Literature summary for 4.2.2.2 extracted from

  • Miyairi, K.; Nishida, K.; Takarae, M.; Shikanai, Y.; Okuno, T.
    Purification and characterization of pectate lyase I and II from fungus Stereum purpureum (2002), J. Appl. Glycosci., 49, 99-106.
No PubMed abstract available
Search for more literature for 4.2.2.2

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ both pectate lyase I and II require 0.2 mM Ca2+ for maximal activity Chondrostereum purpureum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 x * 48000, SDS-PAGE Chondrostereum purpureum

Organism

Organism UniProt Comment Textmining
Chondrostereum purpureum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Chondrostereum purpureum

Purification (Commentary)

Purification (Comment) Organism
pectate lyase I and II Chondrostereum purpureum

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Chondrostereum purpureum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polygalacturonic acid endo-type reaction Chondrostereum purpureum 4,5-unsaturated digalacturonic acid + 4,5-unsaturated trigalacturonic acid + oligogalacturonic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Chondrostereum purpureum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Chondrostereum purpureum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Chondrostereum purpureum

pI Value

Organism Comment pI Value Maximum pI Value
Chondrostereum purpureum pectate lyase II
-
 4.1
Chondrostereum purpureum pectate lyase I
-
 4.2