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Literature summary for 4.2.2.2 extracted from
- Pectate lyase 10A from Pseudomonas cellulosa is a modular enzyme containing a family 2a carbohydrate-binding module (2001), Biochem. J., 355, 155-165.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Cellvibrio japonicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-values: 0.104 mg/ml for polygalacturonic acid, 0.131 mg/ml for esterified citrus pectin, 1.837 mg/ml for 67% esterified citrus pectin, 1.837 mg/ml for 89% esterified citrus pectin | Cellvibrio japonicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | optimal activity in presence of 2.0-4.0 mM Ca2+. No activity when Ca2+ is replaced with Co2+, Cu2+, Ba2+, Mg2+, Mn2+, Ni2+, Sr2+ or Zn2+ | Cellvibrio japonicus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
x * 38000, N-terminally His-tagged Pel10Acm, SDS-PAGE | Cellvibrio japonicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellvibrio japonicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 38000 Da N-terminally His-tagged Pel10Acm | Cellvibrio japonicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 67% esterified citrus pectin | - |
Cellvibrio japonicus | ? | - |
? | |
| 89% esterified citrus pectin | - |
Cellvibrio japonicus | ? | - |
? | |
| pectin | esterified citrus pectin | Cellvibrio japonicus | unsaturated oligogalacturonides | - |
? | |
| polygalacturonic acid | i.e. pectate, endo-cleavage, the enzyme has a preference for sequences of non-esterified galacturonic acid residues | Cellvibrio japonicus | unsaturated oligogalacturonides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, N-terminally His-tagged Pel10Acm, SDS-PAGE | Cellvibrio japonicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pectate lyase 10A | - |
Cellvibrio japonicus |
| Pel10A | - |
Cellvibrio japonicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 62 | - |
- |
Cellvibrio japonicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 47 | - |
20 min, stable | Cellvibrio japonicus |
| 52 | - |
20 min, 38% loss of activity | Cellvibrio japonicus |
| 57 | - |
20 min, complete inactivation | Cellvibrio japonicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 22 | - |
89% esterified citrus pectin | 37°C | Cellvibrio japonicus | |
| 259.5 | - |
polygalacturonic acid | 37°C | Cellvibrio japonicus | |
| 260 | - |
polygalacturonic acid | 37°C | Cellvibrio japonicus | |
| 265 | - |
67% esterified citrus pectin | 37°C | Cellvibrio japonicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
polygalacturonic acid and esterified citrus pectin | Cellvibrio japonicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | 11 | pH 9.5: about 25% of maximal activity, pH 11.0: about 35% of maximal activity with polygalacturonic acid as substrate | Cellvibrio japonicus |
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Release 2023.1 (January 2023)
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