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Literature summary for 4.2.2.2 extracted from
- Efficient secretory overexpression of Bacillus subtilis pectate lyase in Escherichia coli and single-step purification (2002), Biochem. Eng. J., 12, 175-179.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| pectate lyase gene fused to the pelB gene encoding the periplasmic secretion signal and the fusion gene is expressed under the control of the T7 promoter in a culture of Escherichia coli BL21DE3 | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
IFO3134 | - |
| Bacillus subtilis IFO3134 | - |
IFO3134 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| single-step | Bacillus subtilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Bacillus subtilis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonic acid | - |
Bacillus subtilis | ? | - |
? |  |
| polygalacturonic acid | - |
Bacillus subtilis IFO3134 | ? | - |
? | |
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Release 2023.1 (January 2023)
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