Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.2.2 extracted from

  • Kobayashi, T.; Koike, K.; Yoshimatsu, T.; Higaki, N.; Suzumatsu, A.; Ozawa, T.; Hatada, Y.; Ito, S.
    Purification and properties of a low-molecular-weight, high-alkaline pectate lyase from an alkaliphilic strain of Bacillus (1999), Biosci. Biotechnol. Biochem., 63, 65-72.
    View publication on PubMed
Search for more literature for 4.2.2.2

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
2,3-Butanedione
-
 Bacillus sp. (in: Bacteria)
2,4,6-Trinitrobenzenesulfonate 1 mM, 73% inhibition Bacillus sp. (in: Bacteria)
Hg2+
-
 Bacillus sp. (in: Bacteria)
N-bromosuccinimide 0.1 mM, 32% inhibition Bacillus sp. (in: Bacteria)
Zn2+
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for polygalacturonic acid: 16% Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20300
-
 analytical ultracentrifugation Bacillus sp. (in: Bacteria)
26000
-
 x * 26000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) KSM-P15
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information protopectinase-like activity on cotton fibers Bacillus sp. (in: Bacteria) ?
-
 ?
additional information protopectinase-like activity on cotton fibers Bacillus sp. (in: Bacteria) KSM-P15 ?
-
 ?
polygalacturonic acid random attack Bacillus sp. (in: Bacteria) unsaturated oligogalacturonate a mixture of 4,5-unsaturated oligogalacturonides, approximately molar ratios of 12:74:14 for monomer, dimer, and trimer, respectively ?
polygalacturonic acid random attack Bacillus sp. (in: Bacteria) KSM-P15 unsaturated oligogalacturonate a mixture of 4,5-unsaturated oligogalacturonides, approximately molar ratios of 12:74:14 for monomer, dimer, and trimer, respectively ?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 30°C: about 25% of maximal activity, 60°C: about 40% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 Ca2+ slightly enhances thermal stability Bacillus sp. (in: Bacteria)
50
-
 30 min, stable up to Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
9.5 11.5 about 30% of maximal activity at pH 9.5 and at pH 11.5 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 30°C, 1 h, with or without CaCl2, stable Bacillus sp. (in: Bacteria)