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Literature summary for 4.2.2.2 extracted from

  • Kobayashi, T.; Hatada, Y.; Higaki, N.; Lusterio, D.D.; Ozawa, T.; Koike, K.; Kawai, S.; Ito, S.
    Enzymatic properties and deduced amino acid sequence of a high-alkaline pectate lyase from an alkaliphilic Bacillus isolate (1999), Biochim. Biophys. Acta, 1427, 145-154.
    View publication on PubMed
Search for more literature for 4.2.2.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
2,3-Butanedione
-
 Bacillus sp. (in: Bacteria)
2-hydroxy-5-nitrophenyl bromide
-
 Bacillus sp. (in: Bacteria)
Fe3+
-
 Bacillus sp. (in: Bacteria)
N-bromosuccinimide
-
 Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ restores activity of the EGTA-inactivated enzyme Bacillus sp. (in: Bacteria)
Mn2+ restores activity of the EGTA-inactivated enzyme Bacillus sp. (in: Bacteria)
Sr2+ restores activity of the EGTA-inactivated enzyme Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33355
-
 x * 33355, calculation from nucleotide sequence Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) KSM-P7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polygalacturonic acid
-
 Bacillus sp. (in: Bacteria) unsaturated oligogalacturonate
-
 ?
polygalacturonic acid
-
 Bacillus sp. (in: Bacteria) KSM-P7 unsaturated oligogalacturonate
-
 ?

Subunits

Subunits Comment Organism
? x * 33355, calculation from nucleotide sequence Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 65
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 stable up to, in absence of CaCl2 Bacillus sp. (in: Bacteria)
60
-
 Ca2+ slightly enhances thermostability up to 60°C Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 12 5°C, 24 h, stable Bacillus sp. (in: Bacteria)