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Literature summary for 4.2.2.2 extracted from
- Polygalacturonic acid trans-eliminase of Xanthomonas campestris (1967), Biochem. J., 104, 178-185.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Xanthomonas campestris |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | up to 1 mM, stimulation | Xanthomonas campestris | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Xanthomonas campestris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Xanthomonas campestris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for a few weeks | Xanthomonas campestris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pentagalacturonic acid | - |
Xanthomonas campestris | saturated digalacturonic acid + unsaturated trigalacturonic acid + saturated trigalacturonic acid + unsaturated digalacturonic acid | preferentially split into saturated digalacturonic acid + unsaturated trigalacturonic acid or into saturated trigalacturonic acid + unsaturated digalacturonic acid - at a lower rate it is also split into monogalacturonic acid and unsaturated tetragalacturonic acid | ? | |
| polygalacturonic acid | - |
Xanthomonas campestris | unsaturated oligogalacturonate | end products at 46% saturation: unsaturated digalacturonic acids, unsaturated trigalacturonic acids, saturated monogalacturonic acid + saturated digalacturonic acid + saturated trigalacturonic acid | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 30 | at pH 9.5 | Xanthomonas campestris |
| 45 | - |
at pH 8.5 | Xanthomonas campestris |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | about 45% of maximal activity at 30°C and at 55°C | Xanthomonas campestris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Xanthomonas campestris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | pH 8: about 35% of maximal activity, pH 10: about 25% of maximal activity | Xanthomonas campestris |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | stable | Xanthomonas campestris |
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Release 2023.1 (January 2023)
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