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Literature summary for 4.2.2.2 extracted from
- Production and properties of polygalacturonate lyase by an alkalophilic microorganism Bacillus sp. RK9 (1978), Can. J. Microbiol., 24, 1164-1172.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 1 mM, complete inhibition | Bacillus sp. (in: Bacteria) |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Bacillus sp. (in: Bacteria) | |
| Ca2+ | optimal concentration: 0.4 mM | Bacillus sp. (in: Bacteria) | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bacillus sp. (in: Bacteria) | constitutive enzyme | ? | - |
? | |
| additional information | Bacillus sp. (in: Bacteria) RK9 | constitutive enzyme | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
RK9 | - |
| Bacillus sp. (in: Bacteria) RK9 | - |
RK9 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | constitutive enzyme | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| additional information | constitutive enzyme | Bacillus sp. (in: Bacteria) RK9 | ? | - |
? | |
| pectic acid | acid soluble | Bacillus sp. (in: Bacteria) | oligouronides of heterogeneous size | - |
? | |
| pectic acid | acid soluble | Bacillus sp. (in: Bacteria) RK9 | oligouronides of heterogeneous size | - |
? | |
| pectic acid amide | about 15% of activity with acid soluble pectic acid | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| pectic acid amide | about 15% of activity with acid soluble pectic acid | Bacillus sp. (in: Bacteria) RK9 | ? | - |
? | |
| pectin | at about 12% of the activity with acid soluble pectic acid | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| pectin | at about 12% of the activity with acid soluble pectic acid | Bacillus sp. (in: Bacteria) RK9 | ? | - |
? | |
| polygalacturonic acid | random attack | Bacillus sp. (in: Bacteria) | unsaturated oligogalacturonate | - |
? | |
| polygalacturonic acid | random attack | Bacillus sp. (in: Bacteria) RK9 | unsaturated oligogalacturonate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | 30°C: 30% of maximal activity, 40°C: 50% of maximal activity, 60°C: optimum | Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
optimal stability, at 65°C | Bacillus sp. (in: Bacteria) |
| 10 | - |
37°C, 1 h, 6% loss of activity | Bacillus sp. (in: Bacteria) |
| 10 | 10.5 | optimal stability, at 45°C-55°C | Bacillus sp. (in: Bacteria) |
| 11 | - |
37°C, 1 h, stable | Bacillus sp. (in: Bacteria) |
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