Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, residues 25-506 corresponding to the full size mature enzyme, labeling and complexing of enzyme in crystals via soaking in cryoprotectant solution containing the heavy atom or disaccharide product in 22.5% w/v PEG 8000, 0.1 M Tris-HCl, pH 8.7, 15% v/v 2-methyl-2,4-pentanediol, and 0.25 M ammonium acetate, equilibration over 1 ml reservoir solution, complexed with disaccharide product, X-ray diffraction structure determination and analysis at 1.7 A resolution, structure modeling of the right-handed parallel beta-helix protein | Pedobacter heparinus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Pedobacter heparinus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pedobacter heparinus | Q46079 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | a heptasaccharide glycosylation site attached to Ser234 | Pedobacter heparinus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[dermatan sulfate]n = [dermatan sulfate]n-1 + 4-deoxy-beta-D-gluc-4-enuronosyl-1,3-GalNAc | acts on dermatan sulfate as sole substrate, catalytic mechanism, Glu333 is involved, substrate binding mechanism involving Arg318 and Arg364 interacting with the sulfate group | Pedobacter heparinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dermatan sulfate | - |
Pedobacter heparinus | unsaturated dermatan sulfate disaccharide | structures | ? |
Synonyms | Comment | Organism |
---|---|---|
chondroitinase B | - |
Pedobacter heparinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8 | broad optimum | Pedobacter heparinus |