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Literature summary for 4.2.2.19 extracted from

  • Huang, W.; Matte, A.; Li, Y.; Kim, Y.S.; Linhardt, R.J.; Su, H.; Cygler, M.
    Crystal structure of chondroitinase B from Flavobacterium heparinum and its complex with a disaccharide product at 1.7 A resolution (1999), J. Mol. Biol., 294, 1257-1269.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, residues 25-506 corresponding to the full size mature enzyme, labeling and complexing of enzyme in crystals via soaking in cryoprotectant solution containing the heavy atom or disaccharide product in 22.5% w/v PEG 8000, 0.1 M Tris-HCl, pH 8.7, 15% v/v 2-methyl-2,4-pentanediol, and 0.25 M ammonium acetate, equilibration over 1 ml reservoir solution, complexed with disaccharide product, X-ray diffraction structure determination and analysis at 1.7 A resolution, structure modeling of the right-handed parallel beta-helix protein Pedobacter heparinus

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Pedobacter heparinus
-
-

Organism

Organism UniProt Comment Textmining
Pedobacter heparinus Q46079
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein a heptasaccharide glycosylation site attached to Ser234 Pedobacter heparinus

Reaction

Reaction Comment Organism Reaction ID
[dermatan sulfate]n = [dermatan sulfate]n-1 + 4-deoxy-beta-D-gluc-4-enuronosyl-1,3-GalNAc acts on dermatan sulfate as sole substrate, catalytic mechanism, Glu333 is involved, substrate binding mechanism involving Arg318 and Arg364 interacting with the sulfate group Pedobacter heparinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dermatan sulfate
-
Pedobacter heparinus unsaturated dermatan sulfate disaccharide structures ?

Synonyms

Synonyms Comment Organism
chondroitinase B
-
Pedobacter heparinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 8 broad optimum Pedobacter heparinus