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Literature summary for 4.2.2.18 extracted from
- Difructose dianhydride III producing inulin fructotransferase from Microbacterium sp. S48-1 (2015), Food Biotechnol., 29, 156-165 .
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5 | - |
inulin | pH 6.0, 60°C | Microbacterium sp. S48-1 |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Microbacterium sp. S48-1 | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
gel filtration | Microbacterium sp. S48-1 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Microbacterium sp. S48-1 | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture fluid | - |
Microbacterium sp. S48-1 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 106 | - |
substrate inulin, pH 6.0, 60°C | Microbacterium sp. S48-1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside | - |
Microbacterium sp. S48-1 | di-D-fructofuranose 1,2':2,3' dianhydride + 1-kestose | - |
? | |
| inulin | - |
Microbacterium sp. S48-1 | di-D-fructofuranose 1,2':2,3' dianhydride + ? | - |
? | |
| additional information | smallest oligosaccharide accepted is nystose | Microbacterium sp. S48-1 | ? | - |
? | |
| nystose | - |
Microbacterium sp. S48-1 | di-D-fructofuranose 1,2':2,3'-dianhydride + sucrose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 45000, SDS-PAGE | Microbacterium sp. S48-1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Microbacterium sp. S48-1 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
30 min, about 80% residual activtiy. Rapid loss of activity above 65°C | Microbacterium sp. S48-1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Microbacterium sp. S48-1 |
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Release 2023.1 (January 2023)
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