Literature summary for 4.2.2.18 extracted from

Zhao, M.; Mu, W.; Jiang, B.; Zhou, L.; Zhang, T.; Lu, Z.; Jin, Z.; Yang, R.
Purification and characterization of inulin fructotransferase (DFA III-forming) from Arthrobacter aurescens SK 8.001 (2011), Biores. Technol., 102, 1757-1764.

Search for more literature for 4.2.2.18

Inhibitors

Inhibitors	Comment	Organism	Structure
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	1 mM, 86% residual activity	Paenarthrobacter aurescens
Cu2+	1 mM, 85% residual activity	Paenarthrobacter aurescens
Fe2+	1 mM, 70% residual activity	Paenarthrobacter aurescens
Mg2+	1 mM, 78% residual activity	Paenarthrobacter aurescens
additional information	not inhibitory at 1 mM: Ba2+, Co2+, Fe3+, Zn2+	Paenarthrobacter aurescens
N-bromosuccinimide	1 mM, 29% residual activity	Paenarthrobacter aurescens
Ni2+	1 mM, 81% residual activity	Paenarthrobacter aurescens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.52	-	inulin	pH 5.5, 60°C	Paenarthrobacter aurescens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	gel filtration	Paenarthrobacter aurescens
40000	-	1 * 40000, SDS-PAGE	Paenarthrobacter aurescens

Organism

Organism	UniProt	Comment	Textmining
Paenarthrobacter aurescens	-	-	-
Paenarthrobacter aurescens SK 8.001	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Paenarthrobacter aurescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
381	-	pH 5.5, 60°C	Paenarthrobacter aurescens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-kestose	-	Paenarthrobacter aurescens	di-D-fructofuranose 1,2':2,3'-dianhydride + D-glucose	di-D-fructofuranose 1,2':2,3'-dianhydride is main product	?
1-kestose	-	Paenarthrobacter aurescens SK 8.001	di-D-fructofuranose 1,2':2,3'-dianhydride + D-glucose	di-D-fructofuranose 1,2':2,3'-dianhydride is main product	?
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside	-	Paenarthrobacter aurescens	di-D-fructofuranose 1,2':2,3'-dianhydride + sucrose	di-D-fructofuranose 1,2':2,3'-dianhydride is main product	?
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside	-	Paenarthrobacter aurescens SK 8.001	di-D-fructofuranose 1,2':2,3'-dianhydride + sucrose	di-D-fructofuranose 1,2':2,3'-dianhydride is main product	?
inulin	-	Paenarthrobacter aurescens	3 di-D-fructofuranose 1,2':2,3' dianhydride + ?	-	?
inulin	-	Paenarthrobacter aurescens SK 8.001	3 di-D-fructofuranose 1,2':2,3' dianhydride + ?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 40000, SDS-PAGE	Paenarthrobacter aurescens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	70	-	Paenarthrobacter aurescens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	4 h, 86.5% residual activity	Paenarthrobacter aurescens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	-	Paenarthrobacter aurescens

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Release 2023.1 (January 2023)