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Literature summary for 4.2.2.18 extracted from

  • Kim, C.S.; Hong, C.K.; Kim, K.Y.; Wang, X.L.; Kang, S.I.; Kim, S.I.
    Cloning, expression, and characterization of Bacillus sp. snu-7 inulin fructotransferase (2007), J. Microbiol. Biotechnol., 17, 37-43.
    View publication on PubMed
Search for more literature for 4.2.2.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a functionally active, His-tagged protein Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 2 * 58000, SDS-PAGE Bacillus sp. (in: Bacteria)
88000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) Q3SAG3 snu-7
-
Bacillus sp. (in: Bacteria) snu-7 Q3SAG3 snu-7
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin
-
 Bacillus sp. (in: Bacteria) 3 di-D-fructofuranose 1,2':2,3' dianhydride + ?
-
 ?
inulin
-
 Bacillus sp. (in: Bacteria) snu-7 3 di-D-fructofuranose 1,2':2,3' dianhydride + ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 58000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
di-D-fructofuranose 1,2': 2,3' dianhydride [DFA III]-producing IFTase
-
 Bacillus sp. (in: Bacteria)
IFTase (DFA III-producing)
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 90% of maximal activity, 70°C: about 80% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 pH 5.5, half-life: 103 min, recombinant and native enzyme Bacillus sp. (in: Bacteria)
70
-
 pH 5.5, half-life: 6.6 min Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
 Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
3 8 pH 3.0: about 40% of maximal activity, pH 8.0: about 55% of maximal activity Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 4°C, 24, stable Bacillus sp. (in: Bacteria)