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Literature summary for 4.2.2.18 extracted from
- Purification and characterization of a di-D-fructofuranose 2',1;2,1'-dianhydride producing enzyme from Streptomyces sp. MCI-2524 (1993), Biosci. Biotechnol. Biochem., 57, 2054-2058.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Streptomyces sp. |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptomyces sp. | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no or little affected by various cations | Streptomyces sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
2 * 36000, SDS-PAGE | Streptomyces sp. |
| 70000 | - |
gel filtration | Streptomyces sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces sp. | - |
- |
- |
| Streptomyces sp. MCI-2524 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Streptomyces sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 256 | - |
purified enzyme | Streptomyces sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin | cf. EC 2.4.1.200 | Streptomyces sp. | di-D-fructofuranose 1,2':2,1' dianhydride + ? | i.e. DFA I | ? | |
| inulin | cf. EC 2.4.1.200 | Streptomyces sp. MCI-2524 | di-D-fructofuranose 1,2':2,1' dianhydride + ? | i.e. DFA I | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 36000, SDS-PAGE | Streptomyces sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Streptomyces sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
stable up to | Streptomyces sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Streptomyces sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | - |
Streptomyces sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
stable | Streptomyces sp. |
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