Literature summary for 4.2.2.18 extracted from

Jahnz, U.; Schubert, M.; Baars-Hibbe, H.; Vorlop, K.D.
Process for producing the potential food ingredient DFA III from inulin: screening, genetic engineering, fermentation and immobilisation of inulase II (2003), Int. J. Pharm., 256, 199-206.

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Application

Application	Comment	Organism
biotechnology	large scale production of sweet food additive DFA III via recombinant expression in E. coli, genetic engineering of the enzyme for enlarged thermotolerance, immobilization of the enzyme on alginate beads	Arthrobacter sp.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli XL-1 blue	Arthrobacter sp.

Protein Variants

Protein Variants	Comment	Organism
G221R	point-mutation introduced via error-prone PCR, increase in activity of 35%	Arthrobacter sp.
additional information	immobilization of enzyme on alginate beads lowered the activity	Arthrobacter sp.
additional information	genetic engineering of the enzyme for enlarged thermotolerance in large scale production of DFA III, recombinant expression in Escherichia coli, elimination of sequence coding for a signal-peptide: 18% secretion, 82% intracellular localisation of the enzyme in Escherichia coli	Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
inulin	Arthrobacter sp.	-	3 di-D-fructofuranose 1,2':2,3' dianhydride + ?	-	?

Organism

Organism	UniProt	Comment	Textmining
Arthrobacter sp.	-	ift gene	-

Purification (Commentary)

Purification (Comment)	Organism
large scale, flocculation from fermenter broth after disruption of recombinant Escherichia coli cells, expressing the enzyme intracellularly	Arthrobacter sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
inulin	-	Arthrobacter sp.	3 di-D-fructofuranose 1,2':2,3' dianhydride + ?	-	?
inulin	-	Arthrobacter sp.	3 di-D-fructofuranose 1,2':2,3' dianhydride + ?	i.e. DFA III	?

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Release 2023.1 (January 2023)