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Literature summary for 4.2.2.18 extracted from

  • Sakurai, H.; Yokota, A.; Tomita, F.
    Molecular cloning of an inulin fructotransferase (depolymerizing) gene from Arthrobacter sp. H65-7 and its expression in Escherichia coli (1997), Biosci. Biotechnol. Biochem., 61, 87-92.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
functional expression of gene ift, strain H65-7, in Escherichia coli strain JM109, DNA sequence determination and analysis, most of the enzyme activity is intracellular Arthrobacter sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Arthrobacter sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid sequence, comparison Arthrobacter globiformis
additional information
-
amino acid sequence, comparison Arthrobacter sp.
49000
-
x * 49000, SDS-PAGE Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis
-
-
-
Arthrobacter globiformis S14-3
-
-
-
Arthrobacter sp.
-
gene ift
-

Purification (Commentary)

Purification (Comment) Organism
from strain H65-7 Arthrobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Arthrobacter sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin
-
Arthrobacter globiformis 3 di-D-fructofuranose 1,2':2,3' dianhydride + ?
-
?
inulin
-
Arthrobacter sp. 3 di-D-fructofuranose 1,2':2,3' dianhydride + ? i.e. DFA III ?
inulin
-
Arthrobacter sp. 3 di-D-fructofuranose 1,2':2,3' dianhydride + ? + oligosaccharides, small amount ?
inulin
-
Arthrobacter globiformis S14-3 3 di-D-fructofuranose 1,2':2,3' dianhydride + ?
-
?
inulin cf. EC 2.4.1.200 Arthrobacter globiformis di-D-fructofuranose 1,2':2,1' dianhydride + ? i.e. DFA I ?
inulin cf. EC 2.4.1.200 Arthrobacter sp. di-D-fructofuranose 1,2':2,1' dianhydride + ? i.e. DFA I ?
inulin cf. EC 2.4.1.200 Arthrobacter globiformis S14-3 di-D-fructofuranose 1,2':2,1' dianhydride + ? i.e. DFA I ?

Subunits

Subunits Comment Organism
? x * 49000, SDS-PAGE Arthrobacter sp.