Cloned (Comment) | Organism |
---|---|
subcloned into a high-expression vector, pET-29b, and the recombinant enzyme with a tag of six histidine is overexpressed in Escherichia coli | Microbacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for levan: 2 mg/ml at pH 7.0 and 40°C | Microbacterium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Microbacterium sp. | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
x * 54000, SDS-PAGE | Microbacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microbacterium sp. | Q9EVQ9 | AL-210 | - |
Microbacterium sp. AL-210 | Q9EVQ9 | AL-210 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Microbacterium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
145.2 | - |
- |
Microbacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
levan | - |
Microbacterium sp. | 5 di-D-fructose-2,6':2',6 dianhydride + ? | - |
? | |
levan | - |
Microbacterium sp. AL-210 | 5 di-D-fructose-2,6':2',6 dianhydride + ? | - |
? | |
additional information | the enzyme never hydrolyzes beta-2,1-fructosyl-linked fructans, such as inulin, as well as oligosaccharides such as 1-kestose and nystose | Microbacterium sp. | ? | - |
? | |
additional information | the enzyme never hydrolyzes beta-2,1-fructosyl-linked fructans, such as inulin, as well as oligosaccharides such as 1-kestose and nystose | Microbacterium sp. AL-210 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, SDS-PAGE | Microbacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
LftM | - |
Microbacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Microbacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9 | about 50% of maximal activity at pH 4.5 and pH 9.0 | Microbacterium sp. |