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Literature summary for 4.2.2.16 extracted from
- Purification of Levan Fructotransferase from Arthvobacter nicotinovoram GS-9 and Production of DFA IV from Levan by the Enzyme. (1997), Biosci. Biotechnol. Biochem., 61, 1705-1709.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Paenarthrobacter nicotinovorans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
gel filtration | Paenarthrobacter nicotinovorans |
| 52000 | - |
1 * 52000, SDS-PAGE | Paenarthrobacter nicotinovorans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| levan | Paenarthrobacter nicotinovorans | the enzyme is specifically induced by levan | 5 di-D-fructose-2,6':2',6 dianhydride + ? | - |
? |  |
| levan | Paenarthrobacter nicotinovorans GS-9 | the enzyme is specifically induced by levan | 5 di-D-fructose-2,6':2',6 dianhydride + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenarthrobacter nicotinovorans | - |
- |
- |
| Paenarthrobacter nicotinovorans GS-9 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Paenarthrobacter nicotinovorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| levan | - |
Paenarthrobacter nicotinovorans | 5 di-D-fructose-2,6':2',6 dianhydride + ? | di-D-fructose-2,6':2',6 dianhydride is the main product, 75%. Fructose, levanbiose, and two unidentified oligosaccharides are minor products | ? | |
| levan | the enzyme is specifically induced by levan | Paenarthrobacter nicotinovorans | 5 di-D-fructose-2,6':2',6 dianhydride + ? | - |
? | |
| levan | - |
Paenarthrobacter nicotinovorans GS-9 | 5 di-D-fructose-2,6':2',6 dianhydride + ? | di-D-fructose-2,6':2',6 dianhydride is the main product, 75%. Fructose, levanbiose, and two unidentified oligosaccharides are minor products | ? | |
| levan | the enzyme is specifically induced by levan | Paenarthrobacter nicotinovorans GS-9 | 5 di-D-fructose-2,6':2',6 dianhydride + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 52000, SDS-PAGE | Paenarthrobacter nicotinovorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Paenarthrobacter nicotinovorans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
20 min, 90% of the initial activity remains | Paenarthrobacter nicotinovorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Paenarthrobacter nicotinovorans |
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