Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 100% reduced activity at 1 mM | Sinorhizobium meliloti | |
Ca2+ | 21-30% reduced activity at 1 mM | Sinorhizobium meliloti | |
Cd2+ | 26-29% reduced activity at 1 mM | Sinorhizobium meliloti | |
Cu2+ | 85% reduced activity at 1 mM | Sinorhizobium meliloti | |
EDTA | 21-30% reduced activity at 1 mM | Sinorhizobium meliloti | |
Hg2+ | 94% reduced activity at 1 mM | Sinorhizobium meliloti | |
Li+ | 26-29% reduced activity at 1 mM | Sinorhizobium meliloti | |
Mn2+ | 21-30% reduced activity at 1 mM | Sinorhizobium meliloti | |
additional information | no inhibition by Mg2+ | Sinorhizobium meliloti | |
NaN3 | 26-29% reduced activity at 1 mM | Sinorhizobium meliloti | |
Zn2+ | 59% reduced activity at 1 mM | Sinorhizobium meliloti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
gel filtration | Sinorhizobium meliloti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | - |
strain M5N1CS, NCIMB 40472 | - |
Sinorhizobium meliloti M5N1 CS | - |
strain M5N1CS, NCIMB 40472 | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, 394fold | Sinorhizobium meliloti |
Storage Stability | Organism |
---|---|
-80°C, 50 mM KCl, purified enzyme, stable for several weeks | Sinorhizobium meliloti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-acetylated glucuronan | beta(1-4)-linked | Sinorhizobium meliloti | ? | - |
? | |
3-O-acetylated glucuronan | beta(1-4)-linked | Sinorhizobium meliloti M5N1 CS | ? | - |
? | |
deacetylated polyglucuronate | beta-(1-4)-linked, best substrate, enzyme shows specific endopolyglucuronate lyase activity | Sinorhizobium meliloti | 4,5-unsaturated oligoglucuronates | - |
? | |
deacetylated polyglucuronate | beta-(1-4)-linked, best substrate, enzyme shows specific endopolyglucuronate lyase activity | Sinorhizobium meliloti M5N1 CS | 4,5-unsaturated oligoglucuronates | - |
? | |
additional information | no activity with polyglucuronate methyl ester alpha-(1-4)-linked, hyaluronate beta-(1-3), beta(1-4)-linked, glucogluronan beta-(1-3), beta(1-4)-linked, alpha-L-guluronate blocks alpha-(1-4)-linked, beta-D-mannuronate blocks beta(1-4)-linked, 2,3-di-O-acetylated glucuronan beta(1-4)-linked, glucuronan beta(1-4)-linked | Sinorhizobium meliloti | ? | - |
? | |
additional information | no activity with polyglucuronate methyl ester alpha-(1-4)-linked, hyaluronate beta-(1-3), beta(1-4)-linked, glucogluronan beta-(1-3), beta(1-4)-linked, alpha-L-guluronate blocks alpha-(1-4)-linked, beta-D-mannuronate blocks beta(1-4)-linked, 2,3-di-O-acetylated glucuronan beta(1-4)-linked, glucuronan beta(1-4)-linked | Sinorhizobium meliloti M5N1 CS | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 20000, SDS-PAGE | Sinorhizobium meliloti |
Synonyms | Comment | Organism |
---|---|---|
(1,4)-beta-D-glucuronan lyase | - |
Sinorhizobium meliloti |
endopolyglucuronate lyase | - |
Sinorhizobium meliloti |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Sinorhizobium meliloti |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
50% of maximal activity | Sinorhizobium meliloti |
67 | - |
complete inactivation | Sinorhizobium meliloti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Sinorhizobium meliloti |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sinorhizobium meliloti | isoelectric focusing | - |
4.9 |