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Literature summary for 4.2.2.13 extracted from

  • Lee, S.S.; Yu, S.; Withers, S.G.
    alpha-1,4-glucan lyase performs a trans-elimination via a nucleophilic displacement followed by a syn-elimination (2002), J. Am. Chem. Soc., 124, 4948-4949.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5-fluoro-beta-L-idopyranosyl fluoride
-
Gracilariopsis sp.
acarbose competitive Gracilariopsis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
p-nitrophenyl alpha-D-glucopyranoside
-
Gracilariopsis sp.
28
-
alpha-D-glucopyranosyl fluoride
-
Gracilariopsis sp.

Organism

Organism UniProt Comment Textmining
Gracilariopsis sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucopyranosyl fluoride
-
Gracilariopsis sp. ?
-
?
additional information the enzyme catalyzes the breakdown of alpha-1,4-glucans via a mechanism involving the formation of a covalent glycosyl-enzyme intermediate which then undergoes a syn-elimination Gracilariopsis sp. ?
-
?
p-nitrophenyl alpha-D-glucopyranoside
-
Gracilariopsis sp. ?
-
?

Synonyms

Synonyms Comment Organism
GLase
-
Gracilariopsis sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.38
-
p-nitrophenyl alpha-D-glucopyranoside
-
Gracilariopsis sp.
505
-
alpha-D-glucopyranosyl fluoride
-
Gracilariopsis sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00002
-
acarbose
-
Gracilariopsis sp.
28.3
-
5-fluoro-beta-L-idopyranosyl fluoride
-
Gracilariopsis sp.