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Literature summary for 4.2.2.12 extracted from

  • Yang, F.; Yang, L.; Guo, X.; Wang, X.; Li, L.; Liu, Z.; Wang, W.; Li, X.
    Production and purification of a novel xanthan lyase from a xanthan-degrading Microbacterium sp. strain XT11 (2014), ScientificWorldJournal, 2014, 368434.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.12

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 10 mM, 19% residual activity Microbacterium sp.
Zn2+ 10 mM, 53% residual activity Microbacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM, 194% of initial activity Microbacterium sp.
K+ 10 mM, 196% of initial activity Microbacterium sp.
Li+ 10 mM, 192% of initial activity Microbacterium sp.
Mg2+ 10 mM, 187% of initial activity Microbacterium sp.
Mn2+ 10 mM, 152% of initial activity Microbacterium sp.
Na+ 10 mM, 192% of initial activity Microbacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 gel filtration Microbacterium sp.

Organism

Organism UniProt Comment Textmining
Microbacterium sp.
-
-
-
Microbacterium sp. XT11
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification from culture liquid Microbacterium sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Microbacterium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28.2
-
 pH 6.0, 40°C Microbacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvylated xanthan enzyme is specific on the pyruvated mannosyl residue in the intact xanthan molecule, but about 50% lyase activity remains when xanthan is partially depyruvated Microbacterium sp. pyruvylate mannose + xanthan oligosaccharides
-
 ?
pyruvylated xanthan enzyme is specific on the pyruvated mannosyl residue in the intact xanthan molecule, but about 50% lyase activity remains when xanthan is partially depyruvated Microbacterium sp. XT11 pyruvylate mannose + xanthan oligosaccharides
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 110000, SDS-PAGE Microbacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Microbacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
 Microbacterium sp.

Expression

Organism Comment Expression
Microbacterium sp. expression is inhibited by glucose down
Microbacterium sp. expression is induced by xanthan up