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Literature summary for 4.2.2.12 extracted from

  • Maruyama, Y.; Mikami, B.; Hashimoto, W.; Murata, K.
    A structural factor responsible for substrate recognition by Bacillus sp. GL1 xanthan lyase that acts specifically on pyruvated side chains of xanthan (2007), Biochemistry, 46, 781-791.
    View publication on PubMed
Search for more literature for 4.2.2.12

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of wild-type enzyme, wild type enzyme in complex with ryruvated D-mannose and mutant enzyme R612A, hanging-drop vapor diffusion Bacillus sp. GL1

Protein Variants

Protein Variants Comment Organism
R313A KM-value for xanthan is 1.5fold higher than wild type value. kcat for xanthan is 219fold lower than wild-type value Bacillus sp. GL1
R612A KM-value for xanthan is 11.6fold higher than wild type value. kcat for xanthan is 2.8fold higher than wild-type value Bacillus sp. GL1
Y315F KM-value for xanthan is 1.8fold higher than wild type value. kcat for xanthan is 1.3fold lower than wild-type value Bacillus sp. GL1

Inhibitors

Inhibitors Comment Organism Structure
4,6-O-[(1S)-1-carboxyethylidene]-beta-D-mannopyranose
-
 Bacillus sp. GL1
D-mannose
-
 Bacillus sp. GL1

Organism

Organism UniProt Comment Textmining
Bacillus sp. GL1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli Bacillus sp. GL1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xanthan
-
 Bacillus sp. GL1 D-xylooligosaccharides
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.92
-
 Xanthan 30°C, pH 5.5, mutant enzyme R313A Bacillus sp. GL1
1730
-
 Xanthan 30°C, pH 5.5, Y315F Bacillus sp. GL1
2170
-
 Xanthan 30°C, pH 5.5, wild-type enzyme Bacillus sp. GL1
6120
-
 Xanthan 30°C, pH 5.5, R612A Bacillus sp. GL1

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.21
-
 4,6-O-[(1S)-1-carboxyethylidene]-beta-D-mannopyranose 30°C, pH 5.5, wild type enzyme Bacillus sp. GL1
4 10 D-mannose 30°C, pH 5.5,mutant enzyme R612A Bacillus sp. GL1
19.2
-
 4,6-O-[(1S)-1-carboxyethylidene]-beta-D-mannopyranose 30°C, pH 5.5,mutant enzyme R612A Bacillus sp. GL1
837
-
 D-mannose 30°C, pH 5.5, wild type enzyme Bacillus sp. GL1