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Literature summary for 4.2.2.11 extracted from

  • Zhu, Y.; Wu, L.; Chen, Y.; Ni, H.; Xiao, A.; Cai, H.
    Characterization of an extracellular biofunctional alginate lyase from marine Microbulbifer sp. ALW1 and antioxidant activity of enzymatic hydrolysates (2016), Microbiol. Res., 182, 49-58 .
    View publication on PubMed
Search for more literature for 4.2.2.11

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 1 mM, 72% residual activity Microbulbifer sp. ALW1
Ca2+ 1 mM, 6% residual activity Microbulbifer sp. ALW1
Co2+ 1 mM, 11% residual activity Microbulbifer sp. ALW1
Cu2+ 1 mM, 10% residual activity Microbulbifer sp. ALW1
EDTA 1 mM, 50% residual activity Microbulbifer sp. ALW1
Fe3+ 1 mM, 11% residual activity Microbulbifer sp. ALW1
Ni2+ 1 mM, 42% residual activity Microbulbifer sp. ALW1
Zn2+ 1 mM, 32% residual activity Microbulbifer sp. ALW1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 alginate Km value 1.03 ml/mg/s, pH 7.0, 45°C Microbulbifer sp. ALW1

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Microbulbifer sp. ALW1
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ 1 mM, 115% of initial activity Microbulbifer sp. ALW1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 gel filtration Microbulbifer sp. ALW1

Organism

Organism UniProt Comment Textmining
Microbulbifer sp. ALW1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification by centrifugation, ammonium sulfate fractionation, anion-exchange chromatography and size exclusion chromatography Microbulbifer sp. ALW1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
 Microbulbifer sp. ALW1 unsaturated algino-oligosaccharides
-
 ?
poly(alpha-(1,4)-L-guluronate)
-
 Microbulbifer sp. ALW1 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
poly(beta-(1,4)-D-mannuronate) reaction of EC 4.2.2.3, about 50% of the activity with poly(alpha-(1,4)-L-guluronate) or alginate Microbulbifer sp. ALW1 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 26000, SDS-PAGE Microbulbifer sp. ALW1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Microbulbifer sp. ALW1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 at least 35% of maximum activity Microbulbifer sp. ALW1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 1 h, 68% residual activity Microbulbifer sp. ALW1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
69.4
-
 alginate pH 7.0, 45°C Microbulbifer sp. ALW1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Microbulbifer sp. ALW1

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 at least 30% of maximum activity Microbulbifer sp. ALW1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 alginate kcat/Km value 67.4 mg/ml, pH 7.0, 45°C Microbulbifer sp. ALW1