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Literature summary for 4.2.2.11 extracted from

  • Han, W.; Gu, J.; Cheng, Y.; Liu, H.; Li, Y.; Li, F.
    Novel alginate lyase (Aly5) from a polysaccharide-degrading marine bacterium, Flammeovirga sp. strain MY04 effects of module truncation on biochemical characteristics, alginate degradation patterns, and oligosaccharide-yielding properties (2016), Appl. Environ. Microbiol., 82, 364-374 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.11

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 10 mM, 135% of initial activity Flammeovirga sp. MY04

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Flammeovirga sp. MY04

Protein Variants

Protein Variants Comment Organism
additional information a truncated protein consisting of the catalytic module degrades alginate into unsaturated oligosaccharides with maximal activity at 30°C, 10°C lower than the temperature for wild-type. Thermostability ranges from 0 to 30°C and highest activity is at pH 7.0 Flammeovirga sp. MY04

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, 116% of initial activity Flammeovirga sp. MY04

Organism

Organism UniProt Comment Textmining
Flammeovirga sp. MY04 A0A0M4N5N7
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
264
-
 substrate alginate, truncated protein consisting of the catalytic module, pH 6.0, 40°C Flammeovirga sp. MY04
620
-
 substrate alginate, wild-type, pH 6.0, 40°C Flammeovirga sp. MY04

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate
-
 Flammeovirga sp. MY04 unsaturated algino-oligosaccharides
-
 ?
saturated hexa((1-4)-alpha-L-guluronan)
-
 Flammeovirga sp. MY04 saturated di((1-4)-alpha-L-guluronan) + unsaturated tetra((1-4)-alpha-L-guluronan)
-
 ?
saturated penta((1-4)-alpha-L-guluronan)
-
 Flammeovirga sp. MY04 saturated di((1-4)-alpha-L-guluronan) + unsaturated tri((1-4)-alpha-L-guluronan)
-
 ?

Subunits

Subunits Comment Organism
? x * 61000, calculated from sequence Flammeovirga sp. MY04

Synonyms

Synonyms Comment Organism
AlgL5
-
 Flammeovirga sp. MY04
Aly5
-
 Flammeovirga sp. MY04

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Flammeovirga sp. MY04

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 40 2 h, more than 80% residual activity Flammeovirga sp. MY04

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Flammeovirga sp. MY04

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 2 h, more than 80% residual activity Flammeovirga sp. MY04

pI Value

Organism Comment pI Value Maximum pI Value
Flammeovirga sp. MY04 calculated from sequence
-
 4.1