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Literature summary for 4.2.2.11 extracted from

  • Farrell, E.K.; Tipton, P.A.
    Functional characterization of AlgL, an alginate lyase from Pseudomonas aeruginosa (2012), Biochemistry, 51, 10259-10266.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
 acetylated alginate pH 7.1, 25°C Pseudomonas aeruginosa
0.005
-
 acetylated poly-(beta-(1->4)-D-mannuronan) pH 7.1, 25°C Pseudomonas aeruginosa
0.011
-
 alginate pH 7.1, 25°C Pseudomonas aeruginosa
0.013
-
 poly-(beta-(1->4)-D-mannuronan) pH 7.1, 25°C Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Storage Stability

Storage Stability Organism
-80°C, presence of 10% glycerol, stable for several months Pseudomonas aeruginosa
4°C, stable for 2 weeks Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylated alginate
-
 Pseudomonas aeruginosa ?
-
 ?
acetylated poly-(beta-(1->4)-D-mannuronan)
-
 Pseudomonas aeruginosa ?
-
 ?
alginate
-
 Pseudomonas aeruginosa ?
-
 ?
additional information operates in a processive manner. It is able to catalyze cleavage adjacent to either mannuronate or guluronate residues in alginate Pseudomonas aeruginosa ?
-
 ?
poly-(beta-(1->4)-D-mannuronan) Km value decreases with increasing substrate length, and kcat/Km increases. Oligomers containing fewer than 9-10 residues are not substrates Pseudomonas aeruginosa ?
-
 ?

Synonyms

Synonyms Comment Organism
AlgL
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
 acetylated alginate pH 7.1, 25°C Pseudomonas aeruginosa
1.5
-
 acetylated poly-(beta-(1->4)-D-mannuronan) pH 7.1, 25°C Pseudomonas aeruginosa
32
-
 alginate pH 7.1, 25°C Pseudomonas aeruginosa
32
-
 poly-(beta-(1->4)-D-mannuronan) pH 7.1, 25°C Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
300
-
 acetylated poly-(beta-(1->4)-D-mannuronan) pH 7.1, 25°C Pseudomonas aeruginosa
500
-
 acetylated alginate pH 7.1, 25°C Pseudomonas aeruginosa
2500
-
 poly-(beta-(1->4)-D-mannuronan) pH 7.1, 25°C Pseudomonas aeruginosa
3100
-
 alginate pH 7.1, 25°C Pseudomonas aeruginosa