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Literature summary for 4.2.2.11 extracted from

  • Kim, H.T.; Chung, J.H.; Wang, D.; Lee, J.; Woo, H.C.; Choi, I.G.; Kim, K.H.
    Depolymerization of alginate into a monomeric sugar acid using Alg17C, an exo-oligoalginate lyase cloned from Saccharophagus degradans 2-40 (2012), Appl. Microbiol. Biotechnol., 93, 2233-2239.
    View publication on PubMed

Application

Application Comment Organism
biofuel production Alg17C can be used as the key enzyme to produce alginate monomers in the process of utilizing alginate for biofuels and chemicals production Saccharophagus degradans
synthesis Alg17C can be used as the key enzyme to produce alginate monomers in the process of utilizing alginate for biofuels and chemicals production Saccharophagus degradans

Cloned(Commentary)

Cloned (Comment) Organism
gene alg17C, expression in Escherichia coli strain BL21(DE3) Saccharophagus degradans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79100
-
x * 81600, about, sequence calculation, x * 79100, recombinant enzyme, SDS-PAGE Saccharophagus degradans
81600
-
x * 81600, about, sequence calculation, x * 79100, recombinant enzyme, SDS-PAGE Saccharophagus degradans

Organism

Organism UniProt Comment Textmining
Saccharophagus degradans Q21FJ0 gene alg17C
-
Saccharophagus degradans 2-40 / ATCC 43961 Q21FJ0 gene alg17C
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Alg17C from Escherichia coli strain BL21(DE3) Saccharophagus degradans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information recombinant Alg17C preferentially acts on oligoalginates with degrees of polymerization higher than 2 to produce the alginate monomer, 4-deoxy-L-erythro-5-hexoseulose uronic acid. The enzyme can produce a monomeric sugar acid from alginate by the concerted action of an endo-type alginate lyase and exo-type alginate lyase Alg17C, substrate specificity of Alg17C, overview Saccharophagus degradans ?
-
?
additional information recombinant Alg17C preferentially acts on oligoalginates with degrees of polymerization higher than 2 to produce the alginate monomer, 4-deoxy-L-erythro-5-hexoseulose uronic acid. The enzyme can produce a monomeric sugar acid from alginate by the concerted action of an endo-type alginate lyase and exo-type alginate lyase Alg17C, substrate specificity of Alg17C, overview Saccharophagus degradans 2-40 / ATCC 43961 ?
-
?

Subunits

Subunits Comment Organism
? x * 81600, about, sequence calculation, x * 79100, recombinant enzyme, SDS-PAGE Saccharophagus degradans

Synonyms

Synonyms Comment Organism
Alg17C
-
Saccharophagus degradans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Saccharophagus degradans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 activity range, recombinant enzyme Saccharophagus degradans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Saccharophagus degradans

pH Range

pH Minimum pH Maximum Comment Organism
6
-
highest activity at pH 6.0, which sharply decreases when the pH is higher or lower than pH 6.0 Saccharophagus degradans

General Information

General Information Comment Organism
evolution the enzyme belongs to the the PL-17 family Saccharophagus degradans