Application | Comment | Organism |
---|---|---|
biofuel production | Alg17C can be used as the key enzyme to produce alginate monomers in the process of utilizing alginate for biofuels and chemicals production | Saccharophagus degradans |
synthesis | Alg17C can be used as the key enzyme to produce alginate monomers in the process of utilizing alginate for biofuels and chemicals production | Saccharophagus degradans |
Cloned (Comment) | Organism |
---|---|
gene alg17C, expression in Escherichia coli strain BL21(DE3) | Saccharophagus degradans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79100 | - |
x * 81600, about, sequence calculation, x * 79100, recombinant enzyme, SDS-PAGE | Saccharophagus degradans |
81600 | - |
x * 81600, about, sequence calculation, x * 79100, recombinant enzyme, SDS-PAGE | Saccharophagus degradans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharophagus degradans | Q21FJ0 | gene alg17C | - |
Saccharophagus degradans 2-40 / ATCC 43961 | Q21FJ0 | gene alg17C | - |
Purification (Comment) | Organism |
---|---|
recombinant Alg17C from Escherichia coli strain BL21(DE3) | Saccharophagus degradans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | recombinant Alg17C preferentially acts on oligoalginates with degrees of polymerization higher than 2 to produce the alginate monomer, 4-deoxy-L-erythro-5-hexoseulose uronic acid. The enzyme can produce a monomeric sugar acid from alginate by the concerted action of an endo-type alginate lyase and exo-type alginate lyase Alg17C, substrate specificity of Alg17C, overview | Saccharophagus degradans | ? | - |
? | |
additional information | recombinant Alg17C preferentially acts on oligoalginates with degrees of polymerization higher than 2 to produce the alginate monomer, 4-deoxy-L-erythro-5-hexoseulose uronic acid. The enzyme can produce a monomeric sugar acid from alginate by the concerted action of an endo-type alginate lyase and exo-type alginate lyase Alg17C, substrate specificity of Alg17C, overview | Saccharophagus degradans 2-40 / ATCC 43961 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 81600, about, sequence calculation, x * 79100, recombinant enzyme, SDS-PAGE | Saccharophagus degradans |
Synonyms | Comment | Organism |
---|---|---|
Alg17C | - |
Saccharophagus degradans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Saccharophagus degradans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | activity range, recombinant enzyme | Saccharophagus degradans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Saccharophagus degradans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | - |
highest activity at pH 6.0, which sharply decreases when the pH is higher or lower than pH 6.0 | Saccharophagus degradans |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the the PL-17 family | Saccharophagus degradans |