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Literature summary for 4.2.2.11 extracted from

  • Li, J.; Dong, S.; Song, J.; Li, C.; Chen, X.; Xie, B.; Zhang, Y.
    Purification and characterization of a bifunctional alginate lyase from Pseudoalteromonas sp. SM0524 (2011), Mar. Drugs, 9, 109-123.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.2.11

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, 48% of initial activity Pseudoalteromonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information KM-value for alpha-L-guluronosyl linkage in alginate, 1.086 mg/ml, for poly(alpha-L-guluronic acid), 0.465 mg/ml at pH 8.5, 50°C Pseudoalteromonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudoalteromonas sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 1 mM, 143% of initial activity Pseudoalteromonas sp.
Ca2+ 1 mM, 136% of initial activity Pseudoalteromonas sp.
Co2+ 1 mM, 123% of initial activity Pseudoalteromonas sp.
K+ 1 mM, 117% of initial activity Pseudoalteromonas sp.
Mg2+ 1 mM, 126% of initial activity Pseudoalteromonas sp.
Mn2+ 1 mM, 116% of initial activity Pseudoalteromonas sp.
Na+ 1 mM, 143% of initial activity Pseudoalteromonas sp.
Ni2+ 1 mM, 110% of initial activity Pseudoalteromonas sp.
Sr2+ 1 mM, 124% of initial activity Pseudoalteromonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000, SDS-PAGE Pseudoalteromonas sp.

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas sp.
-
 bifunctional enzyme, activities against poly(beta-D-mannuronate), EC 4.2.2.3, and poly(alpha-L-guluronate), EC 4.2.2.11
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudoalteromonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4803
-
 pH 8.5, 50°C Pseudoalteromonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-L-guluronosyl linkage in alginate
-
 Pseudoalteromonas sp. ? enzyme shows activities toward both polyG, i.e. poly-alpha-(1->4)-L-guluronic acid, and polyM, i.e. poly-beta-D-mannuronic acid ?
poly(alpha-(1->4)-L-guluronic acid)
-
 Pseudoalteromonas sp. ?
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Pseudoalteromonas sp.

Synonyms

Synonyms Comment Organism
Aly-SJ02
-
 Pseudoalteromonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudoalteromonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 half-life 41 min Pseudoalteromonas sp.
50
-
 half-life 20 min Pseudoalteromonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Pseudoalteromonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 most stable at pH 8.0 Pseudoalteromonas sp.