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Literature summary for 4.2.2.11 extracted from

  • Tondervik, A.; Klinkenberg, G.; Aarstad, O.; Drablos, F.; Ertesvag, H.; Ellingsen, T.; Skjak-Brak, G.; Valla, S.; Sletta, H.
    Isolation of mutant alginate lyases with cleavage specificity for Di-guluronic acid linkages (2010), J. Biol. Chem., 285, 35284-35292.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
A78S activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 820 U/mg, against poly(alpha-L-guluronic acid) 938 U/mg. Ratio of activities 1.1 Klebsiella pneumoniae
A78S/T89I activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 228 U/mg, against poly(alpha-L-guluronic acid) 34.3 U/mg. Ratio of activities 0.2 Klebsiella pneumoniae
A78S/T89I/A217E activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 187 U/mg, against poly(alpha-L-guluronic acid) 29.8 U/mg. Ratio of activities 0.2 Klebsiella pneumoniae
G26E activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 692 U/mg, against poly(alpha-L-guluronic acid) 787 U/mg. Ratio of activities 1.1 Klebsiella pneumoniae
G26E/P39H activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 246 U/mg, against poly(alpha-L-guluronic acid) 19.5 U/mg. Ratio of activities 0.1. In the absence of Ca2+, no detectable activity against G-M linkages Klebsiella pneumoniae
G304V activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 828 U/mg, against poly(alpha-L-guluronic acid) 878 U/mg. Ratio of activities 1.1 Klebsiella pneumoniae
I51M activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 731 U/mg, against poly(alpha-L-guluronic acid) 786 U/mg. Ratio of activities 1.1 Klebsiella pneumoniae
I51M/T89I activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 227 U/mg, against poly(alpha-L-guluronic acid) 18.8 U/mg. Ratio of activities 0.1 Klebsiella pneumoniae
I51M/T89I/G304V activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 153 U/mg, against poly(alpha-L-guluronic acid) 13.3 U/mg. Ratio of activities 0.1 Klebsiella pneumoniae
additional information engineering of different lyases, each of which cleaves only one of the four possible linkages in alginates: G-G, G-M, M-G, and M-M. The substitutions conferring altered specificity to the mutant enzymes are located in conserved regions in the polysaccharide lyase family 7 alginate lyases. Structure-function analyses suggests that the improved G-G specificity might be caused by increased affinity for nonproductive binding of the alternating G-M structure Klebsiella pneumoniae
P39H activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 356 U/mg, against poly(alpha-L-guluronic acid) 37 U/mg. Ratio of activities 0.1 Klebsiella pneumoniae
P39T activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 216 U/mg, against poly(alpha-L-guluronic acid) 71 U/mg. Ratio of activities 0.3 Klebsiella pneumoniae
S35R activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 443 U/mg, against poly(alpha-L-guluronic acid) 448 U/mg. Ratio of activities 1.0 Klebsiella pneumoniae
S35R/P39T activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 32.6 U/mg, against poly(alpha-L-guluronic acid) 6.9 U/mg. Ratio of activities 0.2 Klebsiella pneumoniae
S35R/P39T/A224V activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 43 U/mg, against poly(alpha-L-guluronic acid) 3.4 U/mg. Ratio of activities 0.1 Klebsiella pneumoniae
S37I activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 53.8 U/mg, against poly(alpha-L-guluronic acid) 4.3 U/mg. Ratio of activities 0.1 Klebsiella pneumoniae
S86L activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 31 U/mg, against poly(alpha-L-guluronic acid) 9.1 U/mg. Ratio of activities 0.3 Klebsiella pneumoniae
T85A activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) 24.8 is U/mg, against poly(alpha-L-guluronic acid) 4.4 U/mg. Ratio of activities 0.32 Klebsiella pneumoniae
T89I activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 218 U/mg, against poly(alpha-L-guluronic acid) 31 U/mg. Ratio of activities 0.1 Klebsiella pneumoniae
V6I activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is858 U/mg, against poly(alpha-L-guluronic acid) 851 U/mg. Ratio of activities 1.0 Klebsiella pneumoniae
V6I/T85A activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) is 23.6 U/mg, against poly(alpha-L-guluronic acid) 2.2 U/mg. Ratio of activities 0.1 Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
polysaccharide lyase family 7 lyase
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
765
-
substrate poly(alpha-L-guluronic acid), 22°C, pH 7.5 Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(alpha-(1->4)-L-guluronic acid)
-
Klebsiella pneumoniae ?
-
?
poly(alpha-(1->4)-L-guluronic acid)
-
Klebsiella pneumoniae SM0524 ?
-
?
poly(beta-D-mannuronic acid/alpha-(1->4)-L-guluronic acid) alternating structure of alpha-L-guluronic acid and beta-D-mannuronic acid. In wild-type, ratio of activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) to poly(alpha-L-guluronic acid) is 1.2 Klebsiella pneumoniae ?
-
?
poly(beta-D-mannuronic acid/alpha-(1->4)-L-guluronic acid) alternating structure of alpha-L-guluronic acid and beta-D-mannuronic acid. In wild-type, ratio of activity against poly(beta-D-mannuronic acid/alpha-L-guluronic acid) to poly(alpha-L-guluronic acid) is 1.2 Klebsiella pneumoniae SM0524 ?
-
?

Synonyms

Synonyms Comment Organism
AlyA
-
Klebsiella pneumoniae