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Literature summary for 4.2.2.11 extracted from

  • Singh, R.; Gupta, V.; Kumari, P.; Kumar, M.; Reddy, C.; Prasad, K.; Jha, B.
    Purification and partial characterization of an extracellular alginate lyase from Aspergillus oryzae isolated from brown seaweed (2011), J. Appl. Phycol., 23, 755-762.
No PubMed abstract available
Search for more literature for 4.2.2.11

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 2 mM, 95% residual activity Aspergillus oryzae
Cd2+ 2 mM, 25% residual activity Aspergillus oryzae
EDTA 2 mM, 15% residual activity Aspergillus oryzae
ethylene glycol tetraacetic acid 2 mM, 23% residual activity Aspergillus oryzae
Fe2+ 2 mM, 30% residual activity Aspergillus oryzae
glutathione 2 mM, 23% residual activity Aspergillus oryzae
Hg2+ 2 mM, 10% residual activity Aspergillus oryzae
Ni2+ 2 mM, 65% residual activity Aspergillus oryzae
Sr2+ 2 mM, 70% residual activity Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information KM value for sodium alginate is 21.5 mg/ml Aspergillus oryzae

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl 2 mM, 110% of initial activity, 100 mM, 193% of initial activity Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000 plus x * 50000, SDS-PAGE Aspergillus oryzae
50000
-
 x * 45000 plus x * 50000, SDS-PAGE Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
 isolated from brown seaweed
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
67
-
 pH 6.5, 35°C Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-L-guluronosyl linkage in alginate specific for cleaving at the beta-1,4 glycosidic bond between polyM and polyG blocks of sodium alginate, producing homopolymeric blocks of polyM and polyG. Enzyme is inefficient in the degradation of polyM and polyG Aspergillus oryzae ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000 plus x * 50000, SDS-PAGE Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Aspergillus oryzae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 40 more than 80% of maximum activity Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Aspergillus oryzae